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近交系小鼠6个短串联重复序列位点遗传多态性分析

Analysis on genetic polymorphisms of 6 short tandem repeats loci of inbred mice

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【Author】 SONG Guoying1),YANG Weihong2),WANG Chunyao3),CHEN Huayan4),LIU Hua1),ZHANG Guangzheng2),YANG Jing2)1)Department of Cell Biology and Medical Genetics,College of Basic Medical Sciences,Zhengzhou University,Zhengzhou 450001 2)Department of Forensic Medicine,College of Basic Medical Sciences,Zhengzhou University,Zhengzhou 450001 3)Laboratory Animal Center of Henan Province,Zhengzhou 4500524)Laboratory for Function,College of Basic Medical Sciences,Zhengzhou University,Zhengzhou 450052

【机构】郑州大学基础医学院细胞生物学与遗传学教研室；郑州大学基础医学院法医学教研室；河南省实验动物中心；郑州大学基础医学院机能实验室；

【摘要】目的:分析5品系近交系小鼠6个短串联重复序列(STR)位点的遗传多态性。方法:选择位于3号、6号、7号染色体上的6个STR位点,采用PCR扩增、变性聚丙烯酰胺凝胶电泳结合银染方法对5品系近交系小鼠(BALB/C、豫医无毛小鼠、C57、DBA/2、FVB/NJ)各5只进行遗传多态性分析。结果:不同品系近交系小鼠在6个位点(D3Mit15、D3Mit85、D3Mit21、D6Mit81、D7Mit164、D7Nds1)上均出现一清晰条带,不同品系小鼠之间有4个位点(D3Mit15、D3Mit21、D6Mit81、D7Nds1)表现为多态性,2个位点(D3Mit85、D7Mit164)表现为单态性。结论:筛选出4个近交系小鼠STR多态性位点。更多还原

【Abstract】 Aim: To analyze the genetic polymorphism of six short tandem repeats(STR) loci among five stroin inbred mice.Methods:The genetic polymorphisms of 6 selected STR loci(D3Mit15,D3Mit85,D3Mit21,D6Mit81,D7Mit164,D7Nds1) in chromosomes 3,6 and 7 among five strain inbred mice including BALB/C,YYHL,C57,DBA/2,FVB/NJ,were analyzed by PCR amplification,denaturing polyacrylamide gel electrophoresis and silver staining.Results:A total of 6 loci tested among various inbred mice displayed single allelic gene band.Among different strain mice,4 loci(D3Mit15,D3Mit21,D6Mit81,D7Nds1)displayed polymorphism,2 loci(D3Mit85,D7Mit164) displayed monomorphism.Conclusion:4 polymorphic STR loci are obtained.更多还原

【关键词】短串联重复序列；遗传监测；近交系小鼠；
【Key words】 short tandem repeats； genetic monitoring； inbred mice；

【基金】河南省医学科技创新人才工程基金资助项目20010907

【文献出处】郑州大学学报(医学版) ,Journal of Zhengzhou University(Medical Sciences) , 编辑部邮箱 ,2008年06期

【分类号】R-332
【下载频次】69

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