【摘要】 目的:检测食管鳞状细胞癌(ESCC)组织中趋化因子受体CCR7、磷脂酰肌醇-3-激酶(PI3K)和蛋白激酶B(PKB或Akt)的表达,探讨CCR7与PI3K/Akt通路的关系。方法:采用免疫组织化学法检测62例ESCC、31例癌旁不典型增生及62例正常食管黏膜组织中CCR7、PI3K、Akt蛋白的表达。结果:ESCC组织中CCR7、PI3K及Akt 3个指标蛋白阳性表达率(72.6%、66.1%、59.7%)均高于癌旁不典型增生组织(29.0%、35.5%、12.9%)、正常食管黏膜组织(8.1%、14.5%、8.1%),差异均有统计学意义(P均<0.001)。ESCC组织中CCR7与PI3K、Akt蛋白表达呈正相关(r分别为0.324、0.306,P均<0.05)。ESCC组织中CCR7蛋白表达与组织学分级、浸润深度及淋巴结转移相关(P均<0.05),PI3K蛋白表达与组织学分级及淋巴结转移相关(P均<0.05),Akt蛋白表达与淋巴结转移相关(P<0.05)。结论:ESCC组织中高表达的CCR7可能通过PI3K/Akt通路促进ESCC的侵袭转移。更多还原

【Abstract】 Aim: To examine the expression of the chemokine receptor CCR7,phosphatidylinositol-3-kinase(PI3K),and protein kinase B(PKB or Akt) in esophageal squamous cell carcinoma(ESCC) tissue,and to evaluate the correlation between CCR7 and PI3K/Akt signal transduction pathway.Methods:The protein expressions of CCR7,PI3K,and Akt in 62 cases of ESCC,31 cases of adjacent atypical hyperplasia,and 62 cases of normal mucous tissues were detected by immumohistochemistry.Results: The protein expression rates of CCR7(72.6%),PI3K(66.1%),and Akt(59.7%) in ESCC tissue were higher than those of the adjacent atypical hyperplasia(29.0%,35.5%,and 12.9%) and normal mucous tissues(8.1%,14.5%,and 8.1%)(P<0.001).CCR7 protein expression rate of ESCC tissue was correlated with PI3K and Akt expression rate(r=0.324 and 0.306,P<0.05).The expression rate of CCR7 protein was correlated with the histological grade,infiltrative depth,and lymphatic metastasis in ESCC tissue(P<0.05).The expression rate of PI3K protein was rcorelated with the histological grade and lymphatic metastasis in ESCC tissue(P<0.05).The expression rate of Akt protein was correlated with the lymphatic metastasis in ESCC tissue(P<0.05).Conclusion: High expression of chemokine receptor CCR7 may contribute to the invasion and metastasis of ESCC through PI3K/Akt pathway.更多还原