【摘要】 目的:探讨脑卒中后抑郁患者血浆、脑脊液中神经递质及其代谢产物同时测定的方法。方法:采用高效液相色谱/电化学法分别同时测定血浆、脑脊液中去甲肾上腺素(NE)、肾上腺素(E)、3,4-二羟基苯乙酸(DOPAC)、多巴胺(DA)、高香草酸(HAV)、5-羟吲哚乙酸(5-HIAA)、5-羟色胺(5-HT)的含量。结果及结论:该方法快速、简便、分离良好,回收率为90%～107%,线性范围3.0～720μg/L,检出限为0.04ng(S/N=3)。通过在脑卒中抑郁状态患者的血浆、脑脊液单胺类神经递质测定中的应用,显示本方法能满足检测要求。更多还原

【Abstract】 Aim:To determine monoamine transmitters and their metabolites in patient plasma and CSF.Methods:Which include Norepinephrine(NE),epinrphrine(E),3,4-dihydroxyphenylacetic acid(DOPAC),dopamine hydrochloride(DA),homovanillic acid(HVA),5-hydroxy-3-indolylcaproic acid(5-HIAA) and 5-hydroxytryptamine(5-HT) could be determined by high performance liquid chromatographywith electrochemical.Results and Conclusion:The recoveries of these compounds were between 90% and 107% and the linear range was 3.0～720 μg/L with simultaneously detection and distinctly separated from internal standard(3,4-dihydroxybenzylamine,DHBA).The detection limit was 0.04 ng(S/N=3).This method was applied to determining the monoamines in plasma and CSF in patient with depression,and satisfactory results were achieved.更多还原