【摘要】 目的:探讨戊四氮(PTZ)点燃大鼠海马结构白细胞介素1β(IL-1β)表达的特征及托吡酯(TPM)对其表达的影响。方法:将30只成年健康雄性SD大鼠随机分为3组,每组10只。模型组大鼠胃灌入生理盐水(10mL/kg)1h后腹腔注射10 g/L PTZ生理盐水溶液(35mg/kg);TPM组大鼠按40mg/kg胃灌注10g/L TPM生理盐水溶液,1h后腹腔注射PTZ(剂量同模型组);对照组大鼠按10 mL/kg和3.5 mL/kg分别胃灌注和腹腔注射生理盐水。各组每d给药1次。当模型组大鼠性发作行为达到点燃标准时,对大鼠的行为进行观察和记录,采用免疫组织化学方法对各组大鼠海马结构IL-1β的表达进行检测。结果:实验第4周,模型组大鼠达到点燃标准;而TPM组大鼠最高发作级别为3级,均未达点燃标准;对照组大鼠行为正常。与对照组比较,模型组和TPM组大鼠海马结构IL-1β表达增强(P<0.05);TPM组大鼠海马结构IL-1β的表达较模型组降低(P<0.05)。结论:癫大鼠免疫系统处于活化状态,TPM能降低PTZ点燃大鼠海马结构IL-1β的表达而对癫具有一定的抑制作用。更多还原

【Abstract】 Aim: To explore the expressional character of interleukin-1β(IL-1β) and the effect of topiramate(TPM) on its expression in the hippocampus of rats kindled by pentyleneterazol(PTZ).Methods:Thirty healthy male rats were randomly divided into three groups,ten in each group.The rats of group I were given normal saline 10 mL/kg by gavage 1 h before intraperitoneal injection with 10 g/L PTZ at dose of 35 mg/kg daily.The rats of groupⅡ were given 10 g/L TPM noral saline 40 mg/kg by gavage 1 h before injection of 10 g/L PTZ at dose of 35 mg/kg daily.The rats of group Ⅲ(control group) were given the same amount of normal saline as other groups.When the spasm of rats in group I reached kindling criterion,the behavior was observed and EEG was recorded,and immunohistochemistry was used to detect the IL-1β in the hippocampus of all rats.Results: The rats in group I reached kindling criterion at Week 4,while the spasm scale of the rats in groupⅡ was grade 3.Compared with that of group Ⅲ,IL-1β expression in the hippocampus of group I and groupⅡ significantly increased(P<0.05).Furthermore,the IL-1β expression of group I was higher than that of groupⅡ(P<0.05).Conclusion: The immune system of the epileptic rat was greatly activated,TPM can decrease the expression of IL-1β in the hippocampus of epileptic rats,which might provide some protective action through immunosuppression.更多还原