【摘要】 目的:观察雷公藤甲素(TP)对变应性鼻炎大鼠鼻黏膜组织中核转录因子-κB(NF-κB)、诱导型一氧化氮合酶(iNOS)的作用,探讨TP治疗变应性鼻炎的可能机制。方法:健康SD大鼠40只随机等分为4组:卵清蛋白(OVA)致敏组、TP处理组、地塞米松(DM)处理组和正常对照(SC)组。以OVA致敏法建立变应性鼻炎大鼠模型,HE染色观察鼻黏膜组织病理学变化,免疫组织化学SP法测定鼻黏膜组织中NF-κB、iNOS的表达。结果:SC组病理学未见异常表现;TP处理组和DM处理组可见鼻黏膜轻度肿胀,少量炎性细胞浸润;OVA致敏组可见鼻黏膜高度肿胀,大量炎性细胞浸润。OVA致敏组与其他3组比较,NF-κB、iNOS表达强度差异均有统计学意义(P均<0.01),TP处理组和DM处理组间差异均无统计学意义(P均>0.05)。OVA致敏组大鼠鼻黏膜组织中NF-κB的表达强度与iNOS呈正相关(r=0.818,P<0.01)。结论:TP可通过抑制鼻黏膜组织中NF-κB的活性来抑制iNOS的表达。更多还原

【Abstract】 Aim:To investigate the effects of TP on NF-κB and iNOS in nasal mucosa of allergic rhinitis rat, and discuss the possible mechanism of TP′s therapeutic effect on allergic rhinitis.Methods:A total of 40 healthy SD rats were randomly divided into four groups:OVA group,TP group,DM group,and SC group.Model of allergic rhinitis was established by OVA.The pathologic changes were observed by HE stainning. The expressions of NF-κB and iNOS were examined by SP immunohistochemical analysis.Results:There was no pathologic change in SC group.Nasal mucosa in TP and DM group was swelling, and there were some inflammatory cells. Nasal mucosa in OVA group was highly swelling, and there were lots of inflammatory cells. NF-κB and iNOS expression in OVA group had significant difference compared with other three groups (P<0.01).And no difference was observed between TP and DM groups(P>0.05).The expression of NF-κB in OVA group had positive correlation with the expression of iNOS (r=0.818,P<0.01).Conclusion: TP could inhibit expression of iNOS by restaining the activation of NF-κB.更多还原