【摘要】 目的:检测宫颈癌及子宫颈癌前病变组织中凋亡相关蛋白激酶(DAPK)蛋白的表达情况。方法:用免疫组织化学SP法检测52例宫颈癌、60例宫颈上皮内瘤变(CIN)及正常宫颈鳞状上皮组织中DAPK蛋白的表达。结果:正常宫颈、CIN和宫颈癌组织中DAPK蛋白阳性率分别为100%(20/20)、75.0%(45/60)和40.4%(21/52),差异有统计学意义(χ2=27.310,P<0.05)。CINⅠ组织中DAPK蛋白阳性率高于CINⅢ组织(P<0.05)。DAPK表达与宫颈癌临床分期有关(P<0.05),而与宫颈癌的病理类型、组织学分级和淋巴结转移等因素无关(P>0.05)。结论:DAPK作为一种抑癌基因,可能参与了宫颈癌的发生发展,有可能成为一个有价值的宫颈癌分子标志物。更多还原

【Abstract】 Aim:To detect the expression of death-associated protein kinase (DAPK) protein in cervical carcinoma and cervical intraepithelial neoplasia (CIN) tissue.Methods:The expression of DAPK was detected by immunohistochemistry in 52 cases of cervical cancer and 60 cases of CIN tissue.Results:The positive rate of DAPK protein in normal cervical epithelia, CIN and cervical carcinoma tissue were 100%(20/20),25.05%(45/60) and 40.4%(21/52),respectively, there were significant differences(χ2=27.310,P<0.05).The positive rate of DAPK in CINⅢ tissue was higher than that in CIN Ⅰ tissue(P<0.05). There was statistical association of the positive rate of DAPK protein with the stages of cervical cancer,but not with the pathological categories, histological grades and lymph node status(P>0.05).Conclusion:DAPK protein may play an important role in cervical cancer development and progression.It may be a valuable cervical cancer marker.更多还原