【摘要】 通过MTT比色法和Western blot法检测Na5SeV5O18.3H2O的抗肿瘤活性及作用机理.结果表明,Na5SeV5O18.3H2O(0.625~20 mg.L-1)能抑制K562细胞增殖(p<0.05);药物处理K562细胞24 h,可使胞浆细胞色素C,IκBα含量明显增多,核内NF-κB含量减少.结果提示Na5SeV5O18.3H2O能体外抑制K562细胞增殖,其机理可能与线粒体凋亡和NF-κB/IκBα信号转导有关.更多还原

【Abstract】 Aimed to study the effect and mechanism of selenium compound Na5SeV5O18·3H2O,MTT colorimetric method and Western blot were used to test anti-proliferation activity and the content of cytochrome C、NF-κB and IκBα in K562 cells.The results show that Na5SeV5O18·3H2O 0.625~20 mg·L-1 could significantly inhibit proliferation of K562 cells in vitro in a time and dose-dependent manner,IC50 values were 14.41 and 3.45 mg·L-1 after treated for 48 h and 72 h,respectively,and the intracellular content of cytochrome C and IκBα markedly increased and NF-κB decreased when treatment for 24 h.The results imply Na5SeV5O18·3H2O has anti-tumor activity and the mechanism of which may relate to apoptosis of mitochondria and signal pathway of NF-κB/ IκBα.更多还原