【摘要】 目的研究胰岛素样生长因子-1(IGF-1)对大鼠局灶性脑缺血再灌注后c-fos表达的影响及与缺血时间关系,探讨IGF-1对脑缺血再灌注损伤的保护作用。方法制作SD大鼠大脑中动脉缺血再灌注模型。将55只SD雄性大鼠随机分为假手术组(n=5)、对照组(n=25)、IGF-1治疗组(n=25),其中后2组按缺血再灌时间(6h、12h、1d、3d、7d)不同可分为5个亚组,每组5只,治疗组于缺血2h再灌注1h后经腹腔注入40μg/kg稀释为1 ml的IGF-1,假手术组及对照组同时腹腔注入生理盐水1 ml。以上动物均在再灌注后规定时间点用4%多聚甲醛经心脏灌注固定,取大鼠脑组织,应用免疫组化S-P法和HE染色检测c-fos蛋白表达及脑组织结构病理变化。结果与对照组相比,治疗组大鼠脑组织c-fos表达明显减少,神经细胞坏死程度明显减轻。结论IGF-1在大鼠局灶性脑缺血再灌注损伤中起保护作用,其作用机制包括降低c-fos的表达,发挥神经保护作用。更多还原

【Abstract】 Objective To evaluate the protective effect of insulin-like growthfactor-1(IGF-1) onfocal cerebral ischemia reperfusionin rats.Methods Fifty-five healthy adult of male SD rats were randomly divided into sham operation group(5 cases),model group(25 cases) and IGF-1 group(25 cases),each of model group and IGF-1 group was further dividedinto 5 subgroups according to the duration of reperfusion(6 hours,12 hours,24 hours,3 days,7 days) after two hours of cerebral ischemia.Then the rats in sham operation and model group were injected normal saline and IGF-1 group were injected IGF-1 through abdominal cavity.Brain tissues of rats were used to observe nerve cell injury under light microscope after HE staining,and positive neurons of c-fos were observed by i mmunohistochemical method.Results Compared with model group,the injury degree of neuron greatly reducedin IGF-1 group (P<0.05).At the same ti me,the number of c-fos positive neuron decreased significantly in IGF-1 group compared with model group(P<0.05).Conclusion IGF-1 plays a protective role in rat focal cerebral ischemia-reperfusion injury,the nervous protective mechanismof IGF-1 may be involved in decreasing c-fos activity.更多还原