【摘要】 选取40只SD雌性大鼠使之交配受孕。在确定其怀孕后,30只孕鼠随机分成正常对照组、阳性对照组和试验组,每组10只。试验组大鼠每天按100 mg/kg体重灌喂黄芪多糖(APS),直至试验结束,而对照组大鼠灌喂相应体积的生理盐水(PSS)。孕鼠于产后72 h分别用灭菌PSS和50μg大肠杆菌内毒素经乳头管灌注到大鼠第4对乳腺内。灌注后24 h处死大鼠,取乳腺组织和颈静脉血进行指标测定。结果显示,阳性对照组大鼠乳腺组织中肿瘤坏死因子α(TNF-α)浓度、N-乙酰-β-D-氨基葡萄糖苷酶(NAGase)活性,与正常对照组相比显著升高(P<0.05);试验组大鼠乳腺组织中TNF-α浓度、NAGase活性与阳性对照组相比,显著降低(P<0.05)。结果表明,灌喂APS可以抑制乳腺组织内炎性细胞因子TNF-α的过度释放,对内毒素诱发的大鼠实验性乳腺炎有一定的保护作用。更多还原

【Abstract】 Forty female rats were mated.After pregnancy was confirmed,thirty pregnant SD rats were randomly divided into normal control group(n=10),positive control group(n=10)and treatment group(n=10).Astragalus polysaccharide(100mg per kg per d)or physiological saline solution(PSS)was administrated by mouth to the rats of the treatment group and control group until they were euthanatized.Either sterile PSS or 50μg Escherichia coli endotoxin was inocu-lated into the fourth(abdominal)mammary gland via the teat duct 72 hours after parturition.Exactly 24 hours after inoculation,the rats were euthanatized.In comparison with those of normal control group,tumor necrosis factor-α(TNF-α) concentration and N-acetyl-β-D-glucosamini-dase(NAGase)activity were significantly(P<0.05)higher in the mammary glands of positive control group.While TNF-α concentration and NAGase activity in mammary gland of treatment group significantly(P<0.05)decreased,compared with those of positive control group.The results demonstrated that astragalus polysaccharide can inhibit excessive release of TNF-α in mammary gland,and has protective effect on rat experimental mastitis induced by endotoxin.更多还原