【摘要】 为建立糖橙的高效遗传转化体系,把无融合生殖基因DefH9-iaaM导入到糖橙中,以获得定向改良目的性状的糖橙新种质.以实生态糖橙节间茎段为材料,建立了糖橙的高效再生体系.结果显示,30d左右苗龄的实生态糖橙茎段不定芽再生率较高;暗培养3～6d有利于节间茎段不定芽的分化;不定芽再生的最佳培养基配方为MS附加6-BA5.0mg/L,平均每根茎段可以再生3.3个小芽;MS+6-BA1.0mg/L+NAA0.1mg/L有利于不定芽的增殖;MS附加NAA0.5mg/L有利于再生芽的生根.用农杆菌介导法进行转化后,经PCR鉴定已获得6株转基因株系.更多还原

【Abstract】 A parthenocarpy gene (DefH9-iaaM) was introduced into Succari to obtain possible seedless transformants, by using internodal stem segments as explants to establish an efficient regeneration and transgenic system. The results indicated that 30-day-old seedlings were the most efficient explants. The co-culture in darkness for 3～6 days gave a rise to good regeneration. The optimum medium for adventitious buds regeneration was MS+6-BA 5.0 mg/L. And MS medium supplied with 6-BA1.0 mg/L and NAA0.1 mg/L showed the highest bud multiplication rate,3.3 buds on each internodal segments. The shoots successfully rooted after 20 days in 0.5 mg/L NAA. After Agrobacterium-mediated transformation,6 transgenic plants out of 17 regenerants were identified by PCR.更多还原