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利用栽培稻和普通野生稻BC2F2群体对分蘖数QTLs的动态分析

Dynamic Analysis on Tiller Number QTLs in a BC2F2 Population Between Oryza sativa and O. rufipogon

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【作者】 程桂平冯九焕刘向东刘桂富李金泉

【Author】 CHENG Gui-ping,FENG Jiu-huan,LIU Xiang-dong,LIU Gui-fu,LI Jin-quan(Guangdong Provincial Key Laboratory of Plant Molecular Breeding,College of Agriculture,South China Agricultural University,Guangzhou 510642,China)

【机构】 广东省植物分子育种重点实验室华南农业大学农学院

【摘要】 以广陆矮4号Oryza sativassp.indica为母本,普通野生稻O.rufipogonGriff.为父本杂交和回交构建了1个含122个单株的BC2F2群体,通过对不同发育时期的分蘖数及其增量的QTL定位分析,确定了与水稻分蘖数有关的QTL数量及其表达时期.结果表明,共有9个QTLs与水稻分蘖数有关,它们分布在第1、2、5、8、9和11号染色体的相应标记区间内.各个QTL对分蘖数均表现出加性和显性作用,且多数位点的显性作用强于加性作用.QTL的表达具有明显的阶段性,5个QTLs主要集中在一定时段内表达.研究结果不仅提供了水稻分蘖数QTLs在某个时期的"静态"信息,而且提供了它们在整个发育期的"动态"信息,为有效发掘利用普通野生稻资源中蕴藏的有利分蘖数基因提供了依据.

【Abstract】 A BC2F2 population with 122 lines was developed from a cross and backcross between Guanglu’ai No.4(Oryza sativa ssp.indica) and O.rufipogon.The QTLs linked to rice tiller numbers and their expressing stages were obtained by QTL mapping in different developmental stages and their increment.The results showed that nine QTLs were detected and distributed among the corresponding marker intervals on chromosome 1,2,5,8,9 and 11.Each QTL had additive and dominant effect on tiller numbers,and the dominant effects showed stronger than the additive effects on the majority of QTLs.The QTLs expressed in various stage and five of the QTLs mainly expressed in some specific periods.The results provide not only the "static" information about the QTLs for rice tiller numbers in certain period,but also their "dynamics" information throughout the whole developmental stages,which provide good basis for mining and utilizing the favorable genes for tiller number in O.rufipogon germplasm resources.

【关键词】 栽培稻普通野生稻分蘖数QTL动态分析
【Key words】 Oryza sativaO.rufipogontiller numberQTLdynamic analysis
【基金】 国家自然科学基金-广东省联合基金重点项目(U0631003);广东省自然科学基金(05300831);广东省农业厅和财政厅攻关项目(粤农函[2003]360号);农业部生理生态与遗传育种重点实验室开放课题(200601);江苏省植物功能基因组重点实验室开放课题(KJS03097)
  • 【文献出处】 华南农业大学学报 ,Journal of South China Agricultural University , 编辑部邮箱 ,2008年04期
  • 【分类号】S511.032
  • 【被引频次】7
  • 【下载频次】229
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