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青蒿化感物质的分离与结构鉴定

Allelochemicals Isolation and Structure Identification of Artemisia annua

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【作者】 邓思娟李春远陈实何雪香谷文祥高志梅

【Author】 DENG Si-juan1,LI Chun-yuan1,CHEN Shi1,HE Xue-xiang2,GU Wen-xiang1,GAO Zhi-mei1(1 College of Sciences,South China Agricultural University,Guangzhou 510642,China;2 Guangdong Forest Research Institute,Guangzhou 510520,China)

【机构】 华南农业大学理学院广东省林业科学研究院

【摘要】 对青蒿Artemisia annua地上部分的乙醇提取物进行了系统分离和活性测定.按照溶剂极性不同,将乙醇提取物分成3个部分,并分别进行了生物活性测试,结果表明:石油醚(A)、石油醚-乙酸乙酯混合液[V石油醚∶V乙酸乙酯=1∶1,(B)]和乙酸乙酯(C)提取部分对萝卜、菜心、白菜和水稻的种子发芽和幼苗生长均具有较强的抑制作用,并呈现出低促高抑的现象,抑制活性顺序为C>B>A.在活性追踪下,从C部分分离出6个化合物,并确定了其中5个化合物的结构,分别是青蒿酸、青蒿素B、香豆素、棕榈酸、豆甾醇.

【Abstract】 The allelopathic effects of Artemisia annua were investigated.The experimental results indicated that the petroleum extracts(A),petroleum/ethyl acetate[V∶V=1∶1] extracts(B) and ethyl acetate extracts(C) of Artemisia annua remarkably inhibited the germination and seedling growth of Raphanus sativus,Brassica parachinensis,Brassica perkinensis and Oryza sativa.The inhibitory effects increased with their concentration.The phenomenon that the germination and seedling growth were inhibited at higher concentration while stimulated at lower concentration was also observed.And the order of inhibitions activity is C>B>A.Under the guide of bioassay,six compounds were isolated.Five of them were identified as artemisinic acid,artemisinin B,coumarin,palmitic acid and stigmasterol by using IR,1HNMR,13CNMR and MS.

【关键词】 青蒿化感作用化感物质分离鉴定
【Key words】 Artemisia annuaallelopathyallelochemicalsisolationidentification
【基金】 广东省科技计划项目(2004B50201018)
  • 【文献出处】 华南农业大学学报 ,Journal of South China Agricultural University , 编辑部邮箱 ,2008年03期
  • 【分类号】S482.4
  • 【被引频次】26
  • 【下载频次】481
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