【摘要】 用溶藻弧菌1.1587制备全细胞、全细胞+弗氏完全佐剂(FCA)、脂多糖(LPS)3种疫苗,疫苗经腹腔对黑鲷进行免疫注射,分别在第7、14、28、56 d采鱼血,检测抗体效价、白细胞吞噬活力、溶菌酶活力、SOD活力和抗菌活力;第28 d用5.5×108cfu/mL的溶藻弧菌悬液进行攻毒试验来比较疫苗的免疫效果.结果表明:抗体凝集效价在28 d达到最高值,免疫组2显著高于免疫组1和免疫组3(P<0.05);白细胞吞噬百分数和吞噬指数28 d达到最高值,3个免疫组都显著高于对照组(P<0.05);免疫组1和免疫组2溶菌酶活力在28 d达到最高值,显著高于对照组(P<0.05);SOD活力在28 d达到最高值,免疫组1和免疫组2显著高于对照组(P<0.05).3种疫苗对黑鲷攻毒后20 d的免疫保护率分别为71.4%、85.7%、71.4%.全细胞-FCA疫苗对黑鲷的免疫效果最好.更多还原

【Abstract】 Sparus macrocephalus were vaccinated with a formalin-killed whole-cell vaccine,a whole-cell with Freund’s Complete Adjuvant(FCA) vaccine and a lipopolysacchride vaccine(LPS) isolated from Vibrio alginolyticus via intraperitoneal injection,respectively.Antibody titers,leucocyte phagocytosis,lysozymes,superoxide dismutase and bactericidal activity were examined on 7th,14th,28th,56th d after first-vaccination.A dosage of 0.2 mL of Vibrio alginolyticus 5.5×108 cfu/mL was intraperitoneally injected to the vaccinated fish to evaluate the protective efficiency of the vaccine.The results indicated that:the antibody titers was the highest on 28 d,immunized group 2 was higher significantly than immunized group 1 and 3(P<0.05);The phagocytic percentage(PP) and phagocytic index(PI) were highest on 28 d,and three immunized groups showed significant difference when compared with the control group(P<0.05);Lysozyme of immunization groups 1 and 2 were the highest on 28 d,and showed significant difference compared with control group(P<0.05);SOD was the highest on 28 d,and immunization groups 1 and 2 were higher significantly than the control group(P<0.05).20 d after challenge with Vibrio alginolyticus,the relative percentage survival(RPS) values were 71.4%,85.7%,71.4%,respectively.It showed that the vaccination of a whole-cell with FCA vaccine was the best of all.更多还原