【摘要】 通过对米曲霉ZLF13固态发酵生产真菌α-淀粉酶的培养基要求及发酵工艺条件进行研究,结果表明,以麸皮和适量淀粉为主要原料,添加1号复合无机盐,保持培养基水体积分数为60%～65%,控制培养温度30～34℃,发酵周期65～70 h.中试平均酶活力1 283 U/g.通过对其酶学性质的研究发现,米曲霉ZLF13所产真菌α-淀粉酶最适作用温度为55℃,最适作用pH值为4.8～5.4;65℃以上迅速失活.更多还原

【Abstract】 In this paper,mediums and culture condition in solid-state fermentation for produce of Fungus α-Amylase was studied.The results showed that wheat bran medium,completed starch and compound salt 1 and kept the water content 60 %～65 % and controled the development temperature 30～34 ℃ and kept the ferment period 65～70 h,was utilized to produce Fungus α-Amylase.The average level of producing on the pilot fermentation reached 1 283 U/g during 65～70 h.The properties of Fungus α-Amylase produced by ZLF13 showed that the optimal activity temperature was 55 ℃ and the optimal activity pH was at the range of 4.8～5.4.Most of the enzyme was out of activity quickly when incubating at a certain temperature higher than 65 ℃.更多还原