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大孔树脂对黑加仑果渣花色苷的纯化研究

Study on purification of anthocyanins from blackcurrant marc by macroporous resin

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【作者】 王萍苗雨

【Author】 WANG Ping,MIAO Yu(School of Forestry,Northeast Forestry University,Harbin 150040,China)

【机构】 东北林业大学林学院

【摘要】 确定出X-5大孔树脂为纯化黑加仑果渣花色苷的最佳树脂.最佳纯化条件为径高比1∶20,吸附原液质量浓度34 mg/mL,pH值2.0,吸附体积25 mL,吸附流速1 mL/min,水洗体积4 BV,解吸液乙醇质量分数60%,乙醇体积6 BV,解吸流速1 mL/min,花色苷的收率为89.80%.纯化后花色苷色价为原来的18.26倍.

【Abstract】 This paper confirmes that the best resin to purify anthocyanins from blackcurrant marc is X-5 macroporous resin.The optimum purification conditions are as follows: diameter/height is 1/20,adsorbancy of adsorption liquid is 0.126×102,pH value is 2.0,adsorption volume is 25 mL,flowing rate ether on adsorption or desorption is 1 mL/min,elution of water is 4 column volume,60% ethanol,5 times column volume,the yield reached 89.80%.The color value of purified anthocyanin is 18.26 times higher than that of the begining.

  • 【文献出处】 哈尔滨商业大学学报(自然科学版) ,Journal of Harbin University of Commerce(Natural Sciences Edition) , 编辑部邮箱 ,2008年06期
  • 【分类号】TS264.4
  • 【被引频次】19
  • 【下载频次】382
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