【摘要】 考察青龙衣多糖对S180小鼠红细胞膜组分及流动性的影响.青龙衣多糖对S180小鼠腹腔注射给药7 d,应用聚丙烯酰胺凝胶电泳测定S180小鼠红细胞膜带3蛋白含量,比色法结合试剂盒测定S180小鼠红细胞膜磷脂、胆固醇和唾液酸含量,荧光偏振法结合探针1,6-二苯-1、3、5己三烯测定S180小鼠红细胞膜流动性.青龙衣多糖能增加S180小鼠红细胞膜表面带3蛋白含量(P<0.05);降低S180小鼠红细胞膜Ch/PL值(P<0.05);增加S180小鼠红细胞膜表面唾液酸含量(P<0.05);提高S180小鼠红细胞膜流动性.青龙衣多糖可以改善S180小鼠红细胞膜组分的异常变化,从而保持细胞膜的流动性,在一定程度上恢复红细胞的结构和功能.更多还原

【Abstract】 To study the effect of Qinglongyi polysaccharide on membrane components and fluidity of S180 mice.S180 mice are administrated with Qinglongyi polysaccharide by i.p.for 7d,and determines Band 3 content by SDS-PAGE,uses spectrophotometer with test kit to measure phospholipids,cholesterol and sialic acid,uses fluorescence spectrophotometry with DPH to determine membrane fluidity.It show that Qinglongyi polysaccharide increased Band 3 content of S180 mice(P<0.05),decreases the value of Ch/PL(P<0.05),increases sialic acid content(P<0.05),enhances membrane fluidity(P<0.05).Qinglongyi polysaccharide can adjust the abnormal change of S180 mice,and improves membrane fluidity,restore erythrocyte membrane structure and function to some extent.更多还原