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龙葵碱对HepG₂细胞磷脂酰丝氨酸及断裂DNA研究

Study on PS and cracking DNA in HepG₂ cells induced by solanine

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【Author】 JI Yu-bin1,2,XU Li-li1,2,GAO Shi-yong1,2(1.Postdoctoral Research Station of Institute of Medicine of Center of Research and Development on Life and Environment Science,Harbin University of Commerce,Harbin 150076,China;2.Engineering Research Center of Natural Anticancer Drugs,Ministry of Education,Harbin 150076,China)

【机构】哈尔滨商业大学生命科学与环境科学研究中心药物研究所博士后科研工作站；哈尔滨商业大学生命科学与环境科学研究中心药物研究所博士后科研工作站哈尔滨150076；国家教育部抗肿瘤天然药物工程研究中心；哈尔滨150076；

【摘要】研究龙葵碱对肝癌细胞HepG2的诱导凋亡作用.采用倒置显微镜观察形态学变化,Annexin V-FITC联合PI双染法和Tunel法检测龙葵碱诱导人肝癌HepG2细胞凋亡作用.结果发现龙葵碱作用24 h后,贴壁细胞数量减少、体积缩小,变圆等明显凋亡形态;在激光共聚焦显微镜下观察到AnnexinV-FITC阳性信号为绿色荧光,PI为红色荧光;Tunel阳性信号为绿色荧光.表明龙葵碱通过诱导HepG2细胞凋亡达到抗肿瘤的作用.更多还原

【Abstract】 To investigate the apoptosis effects of solanine in HepG2 cell.The morphology of cells is observed under transmission electron microscopy.Apoptosis of solanine is assessed with Annexin V-FITC /PI and Tunel.The result is that with the inverted microscope,after being treated with drug-solanine,cells grew against the wall and peeled off gradually,which showed the characters of cells apoptosis.With the laser confocal microscopic,the positive signal of Annexin V-FITC is green,and PI is red;the positive signal of tunnel is green.Inhibition on tumor of solanine can be accomplished through inducing the apoptosis in HepG2 cells.更多还原