【摘要】 运用5-氟脲嘧啶制备的小鼠贫血模型研究降解分离后所得阿胶有效组分A、B的升高红、白血球的作用机制。结果显示:体外消化液中提取的A、B组分能促进贫血小鼠外周血白细胞和红细胞的升高,促进骨髓和脾造血干/祖细胞集落BFU-E,CFU-E,CFU-GM的增加,提高外周血GM-CSF,IL-6,EPO的含量,降低负相造血因子INF-γ、TGF-β含量,刺激肝和肾EPO和GM-CSF mRNA表达。从而说明从体外模拟人胃、肠的消化系统分离的阿胶组分A、B能够刺激造血。该有效补血活性成分的升高红、白血球的作用机制可能与保护贫血小鼠造血微环境和造血干、祖细胞,刺激髓外造血器官相关造血细胞因子表达有关。更多还原

【Abstract】 The anemic mice model induced by 5-fluorouracil(5-Fu) was used to explore the possible hematopietic mechanism of fraction A and B from enzymed-digested colla corii asini on erythrocytes and granulocytes.Results show that fraction A and B can improve erythrocytes and granulocytes number in the peripheral blood,protect the hematopoeitc stem cells colonies forming unit BFU-E,CFU-E and CFU-GM in the bone marrow and spleen,enhance the level of GM-CSF and IL-6 and EPO,decrease the level of INF-γ and TGF-β in the serum and stimulate the mRNA expression of GM-CSF and EPO in the kidney and(liver).These results suggest that simulated human gastric and intestinac digestion model is useful for the(separation) of active components from the protein related drugs.The possible hematopoietic mechanism of(colla) corii asini is associated with the protection of hematopoeitic microenviroment and activation of auxi-(liary) hemapoietic organs,liver and spleen and kidney.更多还原