【摘要】 目的探讨葡多酚（GPC）和硫辛酸（LA）对⁶⁰Coγ射线照射小鼠肝细胞导致自由基损伤的拮抗作用。方法将10只1周龄清洁级昆明种小鼠肝脏制成1×10⁹个几肝细胞培养物,细胞存活率均>85%。将肝细胞培养液分为阴性对照组(未经⁶⁰Coγ线照射)、GPC 组（终浓度为75 mg/L）、LA 组（终浓度为100 mg/L）和阳性对照组（不含任何抗氧化剂）,GPC组、LA 组和阳性对照组给予剂量为2.5 Gy 的⁶⁰Coγ射线照射,距离90 cm,持续10 s。检测肝细胞超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）活力和丙二醛（MDA）含量,并进行彗星试验。结果与阳性对照组比较,GPC 组和LA 组肝细胞 SOD、GSH-Px 活力均较高（P<0.05或 P<0.01）,MDA 含量均较低（P<0.01）,彗星面积较小（P<0.05）,尾长较短（P<0.01）,拖尾率较低（P<0.05和 P<0.01）。结论葡多酚和硫辛酸对⁶⁰Coγ射线照射小鼠肝细胞导致的自由基损伤有拮抗作用。更多还原

【Abstract】 Objective To study the antagonistic effects of grape procyanidin（GPC）or lipoic acid（LA）to the rat liver cell peroxidative damage induced by⁶⁰Co γ radiation.Methods The cultured rat liver cells（1×10⁹/L）were prepared from 10 rats aged 1 week,the survival rate was above 85%.The cultured liver cells were divided into the negative control group（without radiation）, GPC group（75 mg/L）,LA group（100 mg/L）and the positive control group（containing no antioxidants）,which were radiated by （60）^Co γ radiation（2.5 Gy,90 cm,10 s）.The SOD,GSH-Px activity,MDA level and comet assay were determined.Results Compared with the positive control group,in the GPC group and LA group,the activity of hepatic SOD and GSH-Px were significantly higher （P<0.05 and P<0.01）respectively,the MDA level was significantly lower（P<0.01）,the comet field and tail length were significantly lower（P<0.05 and P<0.01）respectively.Conclusion Grape procyanidin or lipoic acid has a significant antagonistic effect against the liver cell peroxidative damage induced by （60）^Co γ radiation.更多还原