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葡多酚和硫辛酸对60Coγ射线致小鼠肝细胞自由基损伤的拮抗作用
Antagonistic Effect of Grape Procvanidin or Linoic Acid to Rat laver Cell Peroxidative Damage Induced bv 60Co γ Radiation
【摘要】 目的探讨葡多酚(GPC)和硫辛酸(LA)对60Coγ射线照射小鼠肝细胞导致自由基损伤的拮抗作用。方法将10只1周龄清洁级昆明种小鼠肝脏制成1×109个几肝细胞培养物,细胞存活率均>85%。将肝细胞培养液分为阴性对照组(未经60Coγ线照射)、GPC 组(终浓度为75 mg/L)、LA 组(终浓度为100 mg/L)和阳性对照组(不含任何抗氧化剂),GPC组、LA 组和阳性对照组给予剂量为2.5 Gy 的60Coγ射线照射,距离90 cm,持续10 s。检测肝细胞超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力和丙二醛(MDA)含量,并进行彗星试验。结果与阳性对照组比较,GPC 组和LA 组肝细胞 SOD、GSH-Px 活力均较高(P<0.05或 P<0.01),MDA 含量均较低(P<0.01),彗星面积较小(P<0.05),尾长较短(P<0.01),拖尾率较低(P<0.05和 P<0.01)。结论葡多酚和硫辛酸对60Coγ射线照射小鼠肝细胞导致的自由基损伤有拮抗作用。
【Abstract】 Objective To study the antagonistic effects of grape procyanidin(GPC)or lipoic acid(LA)to the rat liver cell peroxidative damage induced by60Co γ radiation.Methods The cultured rat liver cells(1×109/L)were prepared from 10 rats aged 1 week,the survival rate was above 85%.The cultured liver cells were divided into the negative control group(without radiation), GPC group(75 mg/L),LA group(100 mg/L)and the positive control group(containing no antioxidants),which were radiated by (60)Co γ radiation(2.5 Gy,90 cm,10 s).The SOD,GSH-Px activity,MDA level and comet assay were determined.Results Compared with the positive control group,in the GPC group and LA group,the activity of hepatic SOD and GSH-Px were significantly higher (P<0.05 and P<0.01)respectively,the MDA level was significantly lower(P<0.01),the comet field and tail length were significantly lower(P<0.05 and P<0.01)respectively.Conclusion Grape procyanidin or lipoic acid has a significant antagonistic effect against the liver cell peroxidative damage induced by (60)Co γ radiation.
【Key words】 Radiation; Grape procyanidin; Lipoic acid; Lipid peroxidation; Comet assay;
- 【文献出处】 环境与健康杂志 ,Journal of Environment and Health , 编辑部邮箱 ,2008年08期
- 【分类号】R818.7
- 【被引频次】1
- 【下载频次】70