【摘要】 目的了解臭氧-生物活性炭(O3-BAC)深度处理水中有机提取物对DNA的损伤作用。方法于2005年6—7月,采集A水厂O3-BAC处理工艺过程中的原水、前接触水、沙滤水、后接触水、BAC水、出厂水水样,以及B水厂一般处理工艺过程中原水、前接触水、沙滤水、出厂水水样。采用A水厂(7.00、3.00、1.50、1.00、0.75、0.38L/皿剂量组)、B水厂水样有机提取物(7.00、3.00、1.00L/皿剂量组)对沙门组氨酸营养缺陷型TA98、TA100菌株进行Ames试验。采用A水厂水样有机提取物对人外周血进行胞质阻断微核试验(3.00、1.50、0.75、0.38L/皿剂量组),对人外周单核细胞进行彗星试验(3.00、1.50、0.75、0.38L/皿剂量组),对人胚肺成纤维细胞p53进行ELISA试验(3.00、1.00、0.3L/皿剂量组)。结果A水厂原水、前接触水、沙滤水7.00L/皿组TA98-S9和原水、前接触水、沙滤水3.00、7.00L/皿组TA98+S9的回变菌落数是溶剂对照组的2倍;B水厂原水、前接触水、沙滤水7.00L/皿组、出厂水3.00、7.00L/皿组TA98-S9和原水、沙滤水、出厂水3.00、7.00L/皿组、前接触水7.00L/皿组TA98+S9的回变菌落数是溶剂对照组的2倍。A水厂原水、沙滤水3.00L/皿组淋巴细胞微核率高于溶剂对照组(P<0.05)。A水厂原水、前接触水、沙滤水0.75、1.50、3.00L/皿组和后接触水、BAC水、出厂水1.50、3.00L/皿组的彗星尾长大于溶剂对照(P<0.05)。与相同剂量组出厂水比较,沙滤水1.0、3.0L/皿组p53蛋白浓度较高(P<0.05)。结论O3-BAC法深度处理工艺能够降低饮水中的有机提取物对DNA的损伤作用。更多还原

【Abstract】 Objective To understand the effect of the organic extracts of tap water deeply treated with O3-BAC on DNA damage. Methods During June to July 2005, water samples were collected from 6 sites in waterworks A treated with O3-BAC, the raw water, the pre-chlorination water, the filtration water, the post-ozonation water, the BAC water and the tap water respectively and 4 sites in waterworks B treated by general treatment, the raw water, the pre-chlorination water, the filtration water, and the tap water respectively. The test was carried out on extracts of water sample from waterworks A with dosage(7.00, 3.00, 1.50, 1.00, 0.75, 0.38 L/plate)and waterworks B with dosage(7.00, 3.00, 1.00 L/plate)using S.typhimurium strains TA98 and TA100. Cytokinesis-blocked micronucleus (CBMN) test, Comet assay were used on extracts of water sample from waterworks A with dosage(3.00, 1.50, 0.75, 0.38 L/plate). Human embryo lung fibroblast (KMB17 strain) p53 ELISA were used with dosage(3.00, 1.00, 0.3 L/plate). Results Ames test showed that in the waterworks A, at the dose of 7.0 L, the revertants of the raw water, pre-chlorination water, the filtration water on TA98-S9 and 7.0 L, 3.0 L/plate, the revertants of the raw water, pre-ozonation water, filtration water on TA98+S9 were twice more than that of solvent control; in waterworks B, at the dose of 7.0 L/plate, the revertants of the raw water, pre-chlorination water, filtration water on TA98-S9 and 7.0 L, 3.0 L/plate the revertants of the tap water on TA98-S9, and 7.0 L/plate pre-chlorination water on TA98+S9 were twice more than that of solvent control. Cytokinesis-blocked micronucleus (CBMN) test showed that in waterworks A, at the dose of 3.0 L/plate, the micronucleus rates of the raw water, filtration water were significant high than that of solvent control(P<0.05). The comet assay showed that in waterworks A, at the dosage of 3.00, 1.50, 0.75 L/plate, the tail length of the raw water, pre-chlorination water, the filtration water and at the doges of 1.50, 3.00 L/plate, the tail length of the post-ozonation water, the BAC water and the tap water were significant longer than that of solvent control(P<0.05). In ELISA assay, at the dosage of 1.0, 3.0 L/plate, compared with the same dose, the p53 protein concentration of the filtration water was higher than that of the tap water (P<0.05). Conclusions The ozone-biological active carbon (O3-BAC) technique may significantly reduce the effect of organic extracts of tap water on DNA damage.更多还原