【摘要】 以沼泽红假单胞菌W1为研究对象,考察了活性黑5(reactive black 5,RB5)脱色产物对直接黄11(direct yellow 11,DY11)生物脱色的影响,并对其机制进行了分析.结果表明,RB5脱色产物能明显加快DY11生物脱色的速率.初始浓度为200 mg/L的DY11加入RB5脱色产物后脱色动力学常数K值可由17 mg/(L.h)增加到42.5 mg/(L.h),优化RB5脱色产物投量,K值可进一步提高到48.8 mg/(L.h).循环伏安分析结果表明,RB5脱色产物具有电化学活性,可媒介电子的转移,其可逆氧化峰(E0)位于83 mV,可逆还原峰(Er)位于-220 mV.FT-IR和HPLC-MS的分析结果表明,RB5脱色产物中具有电化学活性的物质为TAHNDSDP-1.TAHNDSDP-1具有醌式结构,能通过得失2[H]的方式在氧化态和还原态之间转化,可作为氧化还原介体加速菌株W1对DY11的还原.更多还原

【Abstract】 This study focused on the effects of decolorization products of reactive black 5（RB5） on anaerobic decolorization of direct yellow 11（DY11） by Rhodopseudomonas palustris W1 and its reaction mechanisms.The results showed that the decolorization products of RB5 could obviously accelerate biological decolorization rate of DY11.For initial concentration of 200（mg/L） of DY11,the addition of decolorization products of RB5 resulted in that the decolorization kinetic constant K was increased from 17（mg/（L·h）） to 42.5（mg/（L·h））,followed by an further increase to 48.8（mg/（L·h）） after optimization of RB5 supplement.Cyclic voltammogram analysis demonstrated that the decolorization products of RB5 presented electrochemical activity due to the presence of redox electron transfer mediators,as indicated by an observation of two current peaks of reversible oxidation and reduction appeared at potential of ca.83 mV and-200 mV,respectively.According to LC-MS and FT-IR analysis,the electrochemically activated species of the decolorization products were characterized to be 7-amino-8-hydroxy-1,2-naphthaquinone3,6-disulphonate-1,2-diimine(TAHNDS_DP-1).TAHNDS_DP-1 with quinine-like structure was able to transfer between its oxidative and reductive forms by transferring（2[H]）,which was supposed to be the redox mediator to shuttle electrons from electron donors to azo dyes,thus,improving the reduction of de materials.更多还原