【摘要】 考察改变进水COD浓度(11000、15000、30000 mg/L)或水力停留时间(HRT为42 h、25 h)对模拟高浓度有机废水微好氧连续小试处理效果的影响,并采用荧光原位杂交-流式细胞术(FISH-FCM)、聚合酶链式反应-变性梯度凝胶电泳(PCR-DGGE)等分子生物学技术及Biolog FF微孔板法分析了不同处理阶段稳定期曝气柱污泥的微生态变化,以探讨进水COD浓度或HRT改变时微好氧处理效果变化的微生态机制.结果表明,在全部4个处理阶段,曝气柱污泥中酵母含量均保持在>99.9%的水平.随着进水COD浓度的上升,污泥浓度由2.0 g/L上升到7.3 g/L,COD比去除速度由2.3 kg/(kg.d)下降到1.7 kg/(kg.d),曝气柱污泥真菌群落结构多样性指数由2.05上升至2.19,代谢多样性指数由4.42上升至4.45;而随着HRT下降,污泥浓度从7.3 g/L下降至6.0 g/L,COD比去除速度从1.7 kg/(kg.d)上升至2.8 kg/(kg.d),真菌群落结构多样性指数和代谢多样性指数则分别从2.19和4.45降至0.79和4.36.作为提高进水有机负荷的主要措施,提高进水COD浓度和缩短HRT对于废水处理效果和曝气柱微生态存在截然相反的影响.更多还原

【Abstract】 A lab-scale continuous experiment was designed to investigate the effect of influent COD concentrations((11?000),(15?000),(30?000)(mg/L))or hydraulic retention time(HRT 42,25 h)on the microareobic treatment performance of simulated high-strength organic wastewater.The molecular microbiological technologies,including Fluorescent in-situ hybridization-Flow Cytometry(FISH-FCM),Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis(PCR-DGGE),and Biolog-FF assay method,were used to detect the variation of microbial community in the aerated column during the four pseudo-steady-state periods.The yeast contents remained >99.9% throughout the overall experimental periods according to FISH-FCM.Increasing influent COD concentration brought on a rising MLSS(2.0-7.3(g/L)) and a reduced specific COD removal rate [2.3-1.7(kg/(kg·d))],structural(PCR-DGGE)/metabolic(Biolog FF) diversity index values of fungal community in the aerated column had an increase of(2.05-2.19)/(4.42-4.45).Shortening HRT brought on a reduced MLSS(7.3-6.0(g/L)) and a rising specific COD removal rate [1.7-2.8(kg/(kg·d))],structural(PCR-DGGE)/metabolic(Biolog FF) diversity index values of fungal community had a decrease of(2.19-0.79)/(4.45-4.36).Increasing influent COD concentration or shortening HRT has an absolutely adverse effect on the microaerobic treatment performance and micro-ecology in the aeration column although either of them can increase influent COD loading up to a higher level.更多还原