【摘要】 采用通用引物,通过实时荧光定量PCR方法(QPCR),对西安市5处地表水体中肠道病原细菌的细胞密度进行4个月的连续检测,并将QPCR检测结果与滤膜法测得的大肠菌群CFU值进行比较分析.结果显示,QPCR法可信度为94%,最小检测值为每管未稀释DNA提取物含2.7个大肠杆菌细胞.5个水体(N=60)检测结果表明,QPCR检测结果是大肠菌群CFU的2.2~5倍.病原细菌的几何平均值范围,QPCR法在25~67000 CCE/100 mL之间,MF法大肠菌群为3~45000 CFU/100 mL之间.2种方法的离散和回归分析表明,QPCR检测结果与大肠菌群CFU显著正相关,秩相关系数为r=0.983.更多还原

【Abstract】 A rapid quantitative polymerase chain reaction(QPCR) analysis method with universal primers was developed to detect cell densities of the enteric pathogenic bacteria from 5 surface water of Xi’an City for 4 months continuously.And the detection results by QPCR method were compared with counts of coliforms colony-forming units(CFU) determined by membrane filter(MF) analysis.The results showed that QPCR method had an estimated 94% confidence,and detection limit was 2.7 Escherichia coli cells per sample in undiluted DNA extracts.For five surface waters(N=60),the geometric mean of pathogenic bacteria concentration determined by QPCR was 2.2-5 times of corresponding coliform CFU determined by MF analysis.Using QPCR analysis,these geometric means of pathogenic bacteria concentration ranged from 25(CCE/100 mL) to(67?000)(CCE/100 mL.) Using MF culture analysis,coliforms ranged from 3(CFU/100 mL) to(45?000)(CFU/100 mL.) Regression analysis showed that there was a significant positive correlation between pathogenic bacteria determined by QPCR method and coliforms determined by MF method,the correlation coefficient(r) was 0.983.更多还原