【摘要】 利用分子生物学技术直接从活性污泥样品中提取DNA,采用套式PCR技术对特征基因片断进行扩增,结合DGGE(变性浓度梯度凝胶电泳)分析活性污泥中微生物种群结构.研究了除磷工艺在正常运行情况下的微生态系统种群结构,并分析了微生物群落结构及行为特征.测定了活性污泥中变形杆菌(Proteobacteria)和产酸菌(Acidobacterium)部分菌种的16S rDNA V3区片段序列,通过NCBI(美国国立生物技术信息中心)基因库比对,初步确定了部分细菌的属.在厌氧/好氧和缺氧/好氧工艺中各类优势菌群变化规律研究表明,在除磷效果稳定的情况下,系统中除磷微生物种群结构大致能保持不变,少数数量或种类发生变化的种群与系统中含氧量变化有关,但是处于动态变化中的菌群结构总体能够适应工艺运行环境.更多还原

【Abstract】 It was studied for community structure of microorganisms in the phosphorus removal processes under the circulating situation,and analyzed for microorganism’s structure and behavior characteristics by the molecular biology technique with direct obtaining of DNA from samples of activated sludge,and by nested PCR and DGGE.It was also determined community structure of microorganisms.It was analyzed structures of Proteobacteria and Acidobacterium by 16S rDNA V3 area gene fragments sequences in activated sludge.By comparing gene sequences in the National Center of Biological Information(NCBI),were determined the kinds of part of microorganisms.Analyzing the low of changes of preponderant bacteria in anaerobic/aerobic and anaerobic/anoxic conditions takes to know,that under the stable situation of phosphorus removing,the system of microorganism’s structure can kept mostly constant.Minority races that have changed in amount or kind has something to do with the variation of oxygen level in the system,but structure totally can adapt the environmental conditions of the processes,while it placed in dynamic varieties.更多还原