【摘要】 运用序批式试验,在无分子氧条件下,确定了好氧氨氧化菌的NO2型氨氧化动力学方程,得到了最大氨氧化速率[qNO2,max=0.144 mg.(mg.h)-1]、二氧化氮半饱和常数(KNO2=0.821μmol.L-1)和二氧化氮抑制性常数(Ki=1.721μmol.L-1).在微量NO2气体中添加2%O2氧气后,氨氧化速率明显提高,最大氨氧化速率发生在体积分数2%O2和50×10-6NO2的条件下,达到0.198 mg.(mg.h)-1.在21%O2和微量NO2条件下,氨氧化速率继续大幅度提高;在21%O2和100×10-6NO2时氨氧化速率达到0.477 mg.(mg.h)-1,比无NO2空气曝气条件下氨氧化速率高3倍.提出了NO2表观强化氨氧化函数的概念,建立了在O2和微量NO2混合气体下的氨氧化动力学方程,利用2%O2和微量NO2条件下的实验结果验证了动力学方程,讨论了NO2强化氨氧化的机理.更多还原

【Abstract】 The kinetics of the NO₂-dependent ammonia oxidation was developed for ammonia oxidizer mixed culture when there was no molecular oxygen in the batch tests.The kinetics parameters were determined,where the half saturate coefficient of NO₂ was 0.821(μmol·L^-1),inhibition coefficient of NO₂ concentration was 1.721(μmol·L^-1),and the maximum ammonia oxidation rate were 0.144 (mg·（mg·h）^-1).After adding the volume fraction of O₂ was 2% to trace NO₂,the ammonia oxidation rates increased obviously.The maximum ammonia oxidation rate,0.198 (mg·（mg·h）^-1) occurred under the condition of the mixed gasses containing the volume fraction of O₂ was 2% and 50×10^-6 NO₂.Under the condition of mixed gasses containing the volume fraction of O₂ was 21% to trace NO₂,the ammonia oxidation rates further increased greatly.The maximum ammonia oxidation rate,0.477 (mg·（mg·h）^-1) occurred when the volume fraction of O₂ was 21% and 100×10^-6 NO₂ in the mixed gas,which is 3 times higher than the general aerobic ammonia oxidation rate.The function for NO₂ apparently to enhance ammonia oxidation was suggested.The kinetics model of ammonia oxidation under the conditions of O₂ and trace NO₂ mixed gasses was developed.The model was validated by the results of ammonia oxidation experiments under the conditions of the mixed gasses containing 2% O₂ and trace NO₂.The mechanism for NO₂ to enhance ammonia oxidation under the conditions of O₂ and trace NO₂ mixed gasses was discussed.更多还原