【摘要】 目前异养菌计数是采用国标法——平皿计数法进行的,该法依靠人工观察计数具有一定的主观性、误差大、耗时长、效率低的缺点。为此,采用数字图像处理的方法对工业冷却水中的异养菌数进行检测。将采集到的彩色的菌落图像转换为灰度图像后,首先根据不共线的三个点可以确定一个圆的原理去除了培养皿边缘图像,再利用灰度加权阈值算法对菌落图像进行二值化处理,然后用距离变换和分水岭算法分割粘连的菌落,最后用四邻域搜索的方法对连通区域进行标号后计数。实验证明该方法具有快速、简单、准确等优点,而且计数结果不受菌落形态、大小的影响,是实现菌落计数的一种行之有效的方法。更多还原

【Abstract】 Heterotrophic bacteria is detected and counted by national standard method of China at present,i.e.flat dish numberation,which has the disadvantages of subjectivity,big error and low efficiency because of relying on manual counting.A method of digital image process was developed to determine heterotrophic bacteria in industrial cooling water.After converting the original color image into a greyscale one,we removed the edge image of culture dish on the basis of the principle that three unco-linear points can determine a circle.Then binarization with the grayscale-weighted threshold method,distance transform and watershed algorithm to segment overlapping colonies were applied,and finally four-connected searching algorithm was used to mark and count the colony.It has been proved that the method greatly improves the accuracy and efficiency of the heterotrophic counting and the counting results are not affected by shape or size of the colony.Thus,colony counting based on digital image processing method can be an effective way.更多还原