【摘要】 研究了一株内生真菌Fusarium mairei UH23发酵产紫杉醇的工艺条件。实验结果表明最佳培养条件为:温度26℃,发酵起始pH值7.0,接种量5%,装液量250 mL三角瓶装液60 mL,发酵周期12天。筛选出最佳碳源为葡萄糖,最佳氮源为硝酸铵。在上述条件下发酵,紫杉醇产量达(286.4±20.3)μg/L,较优化前提高10.2%。更多还原

【Abstract】 The fermentation conditions of endophytic fungus Fusarium mairei UH23 were investigated for taxol production.The results showed that an inoculation volume of 5% into the 60 mL/250 mL Erlenmeyer flask at 26 ℃ and initial pH 7.0 could reach the maximum taxol production after 12 days of incubation.Glucose and ammonium nitrate were selected as the best carbon and nitrogen sources in the further study.The taxol yield under the optimized conditions reached(286.4±20.3) μg/L,an increase by 10.2%.更多还原