【摘要】 目的研究脑膜瘤中基质金属蛋白酶2、9(MMP-2、9)及其抑制因子TIMP-1 mRNA表达与其瘤周水肿(PTE)的相关性。方法36例经MRI检查及手术、病理证实的脑膜瘤,根据PTE程度分为2组:无或轻度水肿组和中重度水肿组,将手术标本以免疫组化染色和原位杂交方法对MMP-2、9和TIMP-1 mRNA表达行半定量检测,分析与脑膜瘤瘤周水肿之间的相关性。结果脑膜瘤的瘤周水肿程度与MMP2(P<0.05)和MMP-9(P<0.05)的表达强度差异有统计学意义。而TIMP-1 mRNA的表达在无或轻度水肿组和中重度水肿组之间差异无统计学意义(P>0.05)。结论MMP-2、9的表达及MMP/TIMP比例失衡在脑膜瘤的高侵袭性和瘤周水肿程度的发生中起关键作用,PTE作为脑膜瘤MRI影像的一个重要特征,因反映了肿瘤的侵袭程度,故可作为临床预后评价的指标之一。更多还原

【Abstract】 Objective To determine whether the expressions of Matrix Metalloproteinases-2、9(MMP-2、9) and Tissue Inhibitor of Metalloproteinase-1(TIMP-1) mRNA are correlated with the degree of peritumoral edema(PTE) in human meningioma.Methods 36 meningioma specimens were collected for the retrospective analysis,which were testified by MRI,and proved by operation and pathology.According to the degrees of PTE,the patients were divided into 2 groups: one with minimum to mild edema and the other with moderate to mild edema.The expressions of MMP-2、9 and TIMP 1mRNA level were semi-quantified in tissue sections by immunohistochemical techniques and in situ hybridization respectively,the results of staining were compared with the degrees of PTE.Results A statistical significance was found between the expressions of MMP-2(P=0.012) 、MMP-9(P=0.005)and the degree of PTE in meningioma.No statistical significance between the expression of TIMP-1mRNA and degree of PTE.Conclusion MMP-2、9 as well as MMPs/TIMP ratio may play an important role in the invasion of meningioma and its PTE degrees.In addition,as one of the most valuable MRI characteristics of meningioma,PTE may predict the prognosis of meningiomas.更多还原