【摘要】 目的探讨原因不明复发性流产患者外周血单核细胞共刺激分子B7-1、B7-2、辅助性T淋巴细胞1(Th1)及辅助性T淋巴细胞2(Th2)的表达水平,分析B7-1、B7-2与Th1/Th2比率的关系。方法流式法检测20例原因不明复发性流产女性(流产组)、19例正常妊娠女性(正常妊娠组)和15例正常未孕女性(正常未孕组)的Th1/Th2和B7-1、B7-2的表达水平。结果流产组Th1/Th2(15.8±4.2)、B7-1的表达水平(11.4±1.6)%,显著高于其余2组(P<0.01);流产组B7-2的表达水平(17.5±2.0)%,显著低于其余2组(P<0.01);流产组Th1/Th2与B7-1的表达水平之间存在显著正相关(r=0.9136,P<0.01);流产组Th1/Th2与B7-2的表达水平之间存在显著负相关(r=-9571,P<0.01)。结论原因不明复发性流产患者B7-1、B7-2、Th1及Th2的表达水平显著异常,B7-1、B7-2与Th1/Th2比率显著相关,可能是原因不明复发性流产发生的重要原因。更多还原

【Abstract】 Objective To evaluate the relationship between Th1/Th2 and B7-1,B7-2 in peripheral blood in unexplained recurrent spontaneous abortion(URSA)and to investigate the function of them in the pathogenesis.Methods The expression of Th1/Th2 and B7-1,B7-2 in peripheral blood from 20 patients with URSA,19 normal pregnancy women and 15 normal non-pregnancy women were determined by double-label flow cytometric technique.Results The expression of Th1/Th2(15.8±4.2)and B7-1(11.4±1.6)% was significantly higher in patients with URSA than that in normal pregnancy women and that normal non-pregnancy women(P<0.01).The expression of B7-2(17.5±2.0)% was significantly lower in patients with URSA than that in normal pregnancy women and normal non-pregnancy women(P<0.01).There was positive correlation between Th1/Th2 and B7-1(r=0.9136,P<0.01),while there was negative correlated between Th1/Th2 and B7-2(r=-9571,P<0.01).Conclusion Overexpression of B7-1 and inhibition of B7-2 lead to the imbalance of Th1/Th2,and it might be the reason of URSA.更多还原