【摘要】 目的观察伊贝沙坦对单侧输尿管梗阻大鼠肾间质中PDGF-BB介导的肾间质纤维化的影响。方法制作大鼠肾间质纤维化模型,随机分为假手术组、模型组和伊贝沙坦治疗组,术后第7、14天分别处死各组中6只大鼠,检测大鼠肾功能、尿β2-微球蛋白,用免疫组化方法测定肾组织中α-平滑肌肌动蛋白(α-SMA)和血小板衍生生长因子(PDGF-BB)蛋白表达,动态观察肾脏病理变化。结果与输尿管梗阻核型(UUO)组比较,治疗组肾间质纤维化明显减轻(P<0.05),肾间质中α-SMA、PDGF-BB表达明显减少(P<0.05)。结论伊贝沙坦可通过下调肾间质PDGF-BB表达减轻肾组织间质纤维化。更多还原

【Abstract】 Objective To investigate the effect of irbesartan on the expression of platelet-derived growth factor-bb(PDGF-BB)and its mechanism of renal interstitial fibrosis following unilateral ureteral obstruction(UUO)in rat kidney.Methods The rat models of renal interstitial fibrosis were established,and sham-operation,irbesartan treatment and control group were set up.6 rats of each group were killed respectively at 7、14 days after surgery.Venous blood sample was collected to measure the levels of 24-hour ureal protein.The pathological changes and expression of PDGF-BB,α-smooth muscle actin(α-SMA)in renal interstitium were observed.Results Irbesartan could significantly decrease the expression of PDGF-BB(P<0.05)and α-SMA(P<0.05)in renal interstitium.Conclusion Irbesartan may play a protective role in the kidney by down-regulating PDGF-BB and alleviating renal interstitial fibrosis following UUO.更多还原