【摘要】 前期研究表明,高度不育的infer1含有arf8-1和fer1-1.利用TAIL-PCR,研究组已经证实fer1-1为一个T-DNA插在At4g33050基因(编码Fer1蛋白)的突变体.于是,研究组从ABRC(the Arabidopsis Biological Re-source Center)购买了At4g33050的另一个T-DNA插入突变体fer1-2.在fer1-2与arf8-1的杂交后代F2中,得到了一个与infer1明显不同的部分雄性不育突变体,命名为infer2,该不育株的所有后代均为部分不育.表型鉴定表明infer2在花发育、花的形态结构、角果的形态结构、植株的营养生长和植株的育性等方面与infer1比较存在明显差异.分子标记分析表明infer2仅含有arf8-1的分子标记而不含有fer1-2的分子标记.因此,可以确定infer2是一个不同于infer1的新的拟南芥雄性不育突变体.infer1和infer2均可作为植物雄性不育分子机制研究的良好材料.更多还原

【Abstract】 Our earlier research indicated that infer1 is a male sterile mutant and has molecular marker of both arf8-1 and fer1-1.Using TAIL-PCR,fer1-1 has been found to be a T-DNA insertion line of At4g33050.Thereupon we purchased another T-DNA insertion line of At4g33050 named as fer1-2 from ABRC(the Arabidopsis Biological Resource Center).In F2 progeny of cross between fer1-2 and arf8-1,we found out a partial male sterile mutant designated as infer2.All progenies of this line are partial sterile.infer2 showed obvious differences from infer1 with respect to development of flowers,morphology and structure of flowers and seliques,vegetative growth and fecundity of mature plant.Results from analysis on molecular marker suggest that infer2 carries arf8-1,but does not carry fer1-2.Hence infer2 is different from infer1 not only in phenotype but also in molecular marker.Taken together,infer2 is a novel male sterile mutant of Arabidopsis.Both infer1 and infer2 could be very useful to reveal the mechanism underlying male sterility of plant.更多还原