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透明颤菌血红蛋白基因克隆及在紫色非硫细菌中的表达

Cloning of vitreoscilla hemoglobin gene and expression in Rhodopseudo palustns

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【Author】 ZHANG Li-guo1,LUO Ren-ming1,ZHOU Ji-ti2,L Hong2,DU Jin-min3,GUO Jian-bo1,LUO Xiao1,WANG Xiao-lei1(1.College of Environmental Science and Engineering,Hebei University of Science and Technology,Shijiazhuang Hebei 050018,China;2.School of Environmental and Biological Science and Technology,Dalian University of Technology,Dalian Liaoning 116012,China;3.College of Biological Science and Engineering,Hebei University of Science and Technology,Shijiazhuang Hebei 050018,China)

【机构】河北科技大学环境科学与工程学院；大连理工大学环境与生命学院；河北科技大学生物科学与工程学院；河北科技大学环境科学与工程学院河北石家庄050018；河北石家庄050018；辽宁大连116012；

【摘要】构建了含有透明颤菌血红蛋白基因的可转化大肠杆菌-紫色非硫细菌穿梭质粒(pHSG-MG-vgb),将其转化到紫色非硫细菌(Rhodopseudo palustns),获得了基因工程菌EPB01。经SDS-PAGE和CO示差分光光谱分析证明,重组菌株表达了有活性的透明颤菌血红蛋白,通过低溶解氧诱导后,基因工程菌EPB01的菌体密度较对照菌增加了24%~32%。更多还原

【Abstract】 An E.coli-Rhodopseudo palustns shuttle plasmid,pHSG-MG-vgb containing vitreoscilla hemoglobin gene was constructed and transformed to Rhodopseudo palustns.As a result genetic Engineering strains EPB01 was obtained.After inducing under low dissolved oxygen conditions,the density of engineering strain increased by 24%～32% compared with that of the control group.The results of SDS-PAGE and carbon monoxide(CO) difference spectrum analysis indicated that the recombinant strain expressed vitreoscilla hemoglobin(VHb) having biological activity.更多还原

【关键词】透明颤菌血红蛋白基因；天然启动子；光合细菌；基因工程菌；低氧诱导；
【Key words】 vitreoscilla hemoglobin gene； native promoter； photosynthetic bacteria； engineering strain； hypoxia induction；

【文献出处】河北工业科技 ,Hebei Journal of Industrial Science and Technology , 编辑部邮箱 ,2008年01期

【分类号】Q78
【被引频次】4
【下载频次】197

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