【摘要】 目的:探讨抑郁对荷瘤大鼠生存期的影响及可能机制。方法:将36只SD大鼠随机分为3组,每组12只,A组:空白组;B组:肿瘤组,接种肿瘤细胞但不给予应激;C组:肿瘤抑郁组,于接种肿瘤细胞前后采用长期不可预见性中等强度应激造成分养大鼠抑郁症模型。观察各组皮下接种Walker-256肉瘤细胞后的生存期及一般状态,并用MTT法观察荷瘤鼠血清对刀豆素A(ConA)诱导的正常淋巴细胞转化的抑制作用,放射免疫法检测各组血清皮质醇水平。结果:C组的一般状态最差。A、B、C 3组平均生存期分别为(60.00±0.00)、(47.67±20.52)、(24.83±17.39)d,3组间差异有统计学意义(P<0.05)。A、B、C 3组大鼠血清对ConA诱导的正常淋巴细胞转化均有抑制作用,抑制率分别为(11.84±5.38)%、(25.61±7.14)%、(39.98±8.52)%,组间差异有统计学意义(P<0.01)。与A、B组相比,C组血清皮质醇显著升高(P<0.05)。结论:抑郁可缩短荷瘤大鼠的生存期,其机制与皮质醇水平升高及其导致的免疫抑制有关。更多还原

【Abstract】 Objective: To establish the animal model of depression associated cancer and discuss the effects of depression on neoplasitc cases with depression and their survival rate.Methods:36 SD rats were randomly divided into three groups with 12 rats in each group;group A-blank group;group B-tumor group(tumor cell inoculation without stimulations);group C-tumor depression group.The animal models were developed as stimulations of depression associated with tumor by separation feeding and chronic unpredictable mild stress and/or their receiving tumor-cell-inoculation.Life expectancy and quality of life of rats with inoculated Walker 256 tumor cells were observed.The Inhibitory effect of serum from depressive rats on lymphocyte proliferation introduced by ConA was measured by MTT assay.The serum-cortisol level of each group was detected by radioimmunity essay.Results:After mild stress,all rats were in depression.The life expectancy and quality of life of depression were the worst in group C.The life expectancy of the three groups were(60.00±0.00),(47.67±20.52),(24.83±17.39)d,with significant difference(P<0.05).The serum from depressive and/or bearing cancer rats inhibited ConA-induced lymphocyte proliferation(P<0.05).The inhibition ratio were group A(11.84±5.38)%,group B(25.61±7.14)% and group C(39.98±8.52)% with statistical difference(P<0.01).Compared with group A and B,group C had higher level of serum-cortisol(P<0.05).Conclusion:The stress depression might induce immune dysfunction by increasing serum-cortisol level of the neoplasitc rats,which can affect life expectancy of neoplasitc rats.更多还原