【摘要】 目的通过检测血清中免疫球蛋白G(IgG)含量及培养外周血单个核细胞并检测诱导细胞产生的IL-4与IFN-γ水平,研究有氧健美操运动对女大学生体液免疫应答及细胞免疫应答的影响。方法从南华大学护理学院2003级随机抽取30名女生,将其随机分成3组,分别设为对照组、实验1组、实验2组。实验组分别每周集中进行1或3次健美操训练。于0、2、4、6、8、10、12W抽血分两管,一管不抗凝血取血清测IgG;另一管抗凝血分离单个核细胞并培养,通过植物血凝素诱导后,利用酶联免疫吸咐试验测定培养上清液中白细胞介素-4(IL-4)与干扰素-γ(IFN-γ)含量;通过SPSS12.0统计软件分析受训前后IgG水平及诱导Th1或Th2分别分泌的IFN-γ或IL-4水平。结果每周锻炼3次的女大学生,血清IgG含量比对照组及每周锻炼1次者高,且在第10周与第12周差异有显著性(p<0.05);IL-4含量在第10、12周增高并维持相对稳定,与对照组差异有显著性(p<0.05);每周只锻炼1次组IL-4含量稍高,与对照组比较差异无显著性(p>0.05)。每周锻炼1次或3次者,IFN-γ含量稍升高,但与对照组比较差异无显著性(p>0.05)。每周锻炼3次者,IFN-γ与IL-4比值在第10、12周降低,但与对照组比较差异无显著性(p>0.05)。结论每周坚持3次健美操有氧运动,能适当提高血清IgG水平,并提高Th2细胞分泌IL-4,增强体液免疫与细胞免疫应答,提高机体免疫力。更多还原

【Abstract】 objective objective To study the effect of aerobic callisthenics exercise on humoral or cellular immune response of university female students by analyzing sera IgG and culturing the peripheral blood mononuclear cells(PBMC) and detecting interleukin-4(IL-4) or interferon-γ(IFN-γ) levels secreting with induced Th2 or Th1 cell.Methods Thirty female students of nursing department of University of South China,who entranced university in 2003,were selected as this study objects that were divided randomly to be as control group,test group1 and test group2.Students in Group1 and 2 took part in aerobic callisthenics exercise once or three times every week.In 0,2,4,6,8,10 or 12th week,IgG quantity was detected by single immunodiffusion.PBMC of every student was cultured and induced to secrete IL-4 or IFN-γwith PHA.The levels of IL-4 or IFN-γof the supernatants of culturing PBMC were measured using enzyme linked immunosorbent assay(ELISA) in the 24h or 48h after PBMC cultured.Results IgG quantities lightly increased until the eighth week,but highly increased in the tenth and twelfth week(p<0.05),and IL-4 levels of the supernatants of culturing PBMC of the students,who played callisthenics exercise three times every week,obviously increased and maintained the levels contrasting to the control group in the tenth and twelfth week(p<0.05).It’s no significant difference between the students of group1,who played callisthenics exercise once a week,and group2.But the IL-4 levels of the students of group 1 were higher(p>0.05).Meanwhile,IFN-γ levels of the students who played callisthenics exercise for once or three times a week were increased,but have no significant meaning contrasting to the control group.The quality of IFN-γ/IL-4 levels decreased in the students of group2,but it has no significant meanings(p>0.05).Conclusion: IgG quantities of the students,who took part in appropriate callisthenics exercise,were increased.The humoral immune responses were probably enhanced.It can promote the IL-4 secreted effect and the cells functions.It also can enhance humoral or cellular immune response of university female students if they play appropriate aerobe callisthenics exercise.更多还原