【摘要】 本文选用低值鱼蛋白进行酰化改性,研究其改性程度与酶解特性(酶解物氨态氮得率,TCA可溶性肽得率及肽分子量分布)间的关系。结果表明:酰化鱼蛋白导致氨态氮得率下降,但有利于TCA可溶性氮得率的提高。最终得到60℃/30min热处理后0.3gSA/gPro改性的鱼蛋白酶解10h,TCA可溶性肽得率最高达38%。更多还原

【Abstract】 Low-cost fish protein was acylated,and then the effect of the acylation degree on the enzymatic hydrolysis of the acylated fish protein,including yield of amino acids and TCA-soluble peptide,and molecular weight distribution of peptide,was studied in this paper. Results showed that acylation significantly reduced the yield of amino acids,but improved the TCA-soluble peptides in the hydrolysates of the fish protein. For the acylation of fish protein,the best pre-incubation temperature and time and the dosage of the acylating reagent SA were 60℃,30 min,and 0.3g /g Pro,respectively,under which the highest yield of TCA soluble peptide reached 38% after hydrolyzing the fish protein for 10 h.更多还原