【摘要】 本文报道了利用组织培养手段快速繁殖三系杂交稻博优64和两系不育系HUTB2S的研究结果.由剥去了内稃和外稃的米粒经无菌发芽获得实生苗,以实生苗为外植体,成功地实现了快速繁殖.在组培条件下,水稻无菌实生苗可以反复分株继代增殖.试验了N6、MS、H、1/2MS、BS、SJ-1等6种基本培养基,用于促进分蘖的效果依次为N6>MS>H>1/2MS>BS>SJ-1.在被试的KT、6-BA和ZEA等3种细胞分裂素类激素中,KT最适合用来促进分蘖.促进分蘖的最适蔗糖浓度范围9%~12%.在高蔗糖浓度(9%~12%)和高KT浓度(8~10mg/L)条件下,水稻无菌苗不仅分蘖多,植株健壮,而且根系发达.水稻组培苗移栽至大田后长势旺盛,长相一致,目前尚未发现明显的性状变异.更多还原

【Abstract】 The rapid propagation of three-line hybrid rice Boyou-64 and the sterile line of two-line,HUTB2S via tissue culture were reported in this paper.The seedlings were obtained through the sterile germination of the brown rice glumes stripped,and the rapid propagation was successfully achieved by taking the stubbles of the sterile seedlings as explants.The rice seedlings could be repeatedly subcultured and proliferated by tissue culture in vitro.Six basic media,i.e.N6、MS、H、1/2MS、BS、SJ-1 were tested,and the order of the effect on tillering is N6>MS>H>1/2MS>BS>SJ-1.Among the three kinds of tested cytokinin(KT,6-BA and ZEA),KT was the optimum hormone for promoting tillering.In the optimum concentration range of sucrose and KT(9%~12%,and 8.0mg/L~10.0mg/L,respectively),not only the sterile rice plantlets are robust,forming lots of tillers,but also the root system are very flourishing.After transplanted to the field,the rice plants via tissue culture grow luxuriously,and the growth phase is even.No obvious characteristics of aberrance are observed.更多还原