【摘要】 目的检测DNA修复酶hOGG1及PARP在原发性肝癌及肝硬化组织中的表达,探讨hOGG1和PARP在HCC和肝硬化组织中的表达特点及其与临床病理的关系。方法应用EnVision免疫组化法检测41例非HCC正常肝组织、99例HCC组织、51例HCC癌旁肝硬化组织中hOGG1和PARP表达情况。结果在HCC组织中hOGG1和PARP在细胞核与细胞质中均有表达,其中hOGG1细胞核阳性分度在正常组、肝硬化组、HCC组之间依次增高(P<0.001);PARP细胞核阳性分度在正常组、肝硬化组、HCC组之间依次降低(P<0.001)。多因素分析显示:hOGG1蛋白在细胞核表达与ALT有关(相对危险度为1.022,P=0.041),hOGG1蛋白在细胞浆表达与总胆红素有关(相对危险度为1.155,P=0.018),PARP蛋白在细胞浆表达与外周血白细胞数(相对危险度为0.757,P=0.037)有关。结论在HCC发生过程中hOGG1蛋白在肝细胞核中的表达可能随着病程进展而增强,PARP蛋白在肝细胞核中的表达可能随着病程进展而降低,可能反应了从肝硬化到肝癌进程中肝细胞内氧化应激状态的改变。更多还原

【Abstract】 Objective To explore the expression and clinicopathological implications of human 8-oxoGuanine DNA glycosylase-1(hOGG1) and poly ADP-ribose polymerase(PARP) in hepatocellular carcinoma(HCC) and hepatic cirrhotic tissues.Methods hOGG1 and PARP expression was detected by immuneohistochemical EnVision method on liver tissues of non-HCC controls(n=41),hepatic cirrhotic(n=51) and HCC patients(n=99).Results There were three types of hOGG1 and of PARP positive staining in HCCs: nuclear,cytoplasmic and mixed.The expression levels of hOGG1 in nucleus was significantly higher in HCCs than that in hepatocirrhosis and controls,and significantly higher in hepatocirrhosis than that in controls(P<0.01).The expression levels of PARP in nucleus was significantly lower in HCCs than that in hepatocirrhosis and controls,and significantly lower in hepatocirrhosis than that in controls(P<0.01).The expression of hOGG1 and of PARP was not associated with the clinicopathological factors in HCCs(P>0.05).Multiplicity indicated that hOGG1 expression in nucleus was related to ALT(OR=1.022,P=0.041),hOGG1 expression in cytoplasm was related to T bili(OR=1.155,P=0.018),PARP expression in cytoplasm was related to peripheral blood leucocytes(OR= 0.757,P=0.037).Conclusion During hepatocarcinogenesis,the expression pattern of hOGG1 and PARP in liver cell nucleus might reflect the status of oxidative stress in each stage thus has etiology implications.更多还原