【摘要】 本研究以反相高效液相色谱为定量分析手段,选用5头患子宫内膜炎的奶牛,通过子宫内灌注盐酸环丙沙星(2.5g/头),研究了盐酸环丙沙星在患子宫内膜炎奶牛体内的药物动力学规律。以二氟沙星为内标,血浆样品经甲醇沉淀蛋白,离心,经针头式过滤器处理,用反相高效液相法测定其中盐酸环丙沙星的浓度。色谱条件为:ODS-1C18柱;测定流动相为0.015mol/L四丁基溴化铵溶液-乙腈(92∶8,V/V),pH为3.0;流速为1.0 ml/min;荧光检测器,激发波长(λex)278 nm,发射波长(λem)465 nm。通过采用MCPKP房室分析程序,分析血中浓度-时间数据,发现有3头奶牛血样药时数据符合无吸收三室开放模型。其血样中主要药动学参数为:T1/2α为0.916 h、T1/2β为49.20 h、AUC高达7.6296 mg/L.h、Clβ为1.582 L/kg.h,β为0.642 h-1。有2头奶牛血样药时数据符合一级吸收二室开放模型。其主要药动学参数为:T1/2α为1.26 h、T1/2β为9.2 h、AUC高达28.336 mg/L.h,β为0.3571 h-1。试验结果表明,盐酸环丙沙星子宫给药吸收快,分布广,消除慢。更多还原

【Abstract】 The pharmacokinetics and residues of ciprofloxacin hydrochloride in the milk were investigated in 5 cows suffered from endometritis following single-intrauterine administration at the dosage of 2.5 per cow.Blood and milk were sampled at different intervals after administration and ciprofloxacin hydrochloride concentrations were determined by RP-HPLC method with a fluorescent detector.Difloxacin was used as intra-standard.The concentration-time data were analyzed with MCPKP program.The ciprofloxacin hydrochloride concentration-time data were fitted to a two-compartment open model with first order absorption in two cows suffered from endometritis after single-intrauterus.The main pharmacokinetics parameters were as follow: T1/2α 1.26 h,T1/2β 9.2 h,AUC 28.336 mg/L·h,β 0.3571 h-1.The ciprofloxacin hydrochloride concentration-time data were fitted to a three-compartment open model with non-absorption in other three cows.The main pharmacokinetics parameters were as follow: T1/2α0.916 h,T1/2β 49.20 h,AUC 7.6296 mg/L·h,Clβ 1.582 L/kg·h,β 0.642 h-1.The results showed that ciprofloxaxin hydrochloride was absorbed fast and distributed extensively,but it was eliminated slowly in the blood after single-intrauterine administration.更多还原