【摘要】 目的对NBT法测定血清果糖胺进行改良。方法针对该法存在的主要问题:校正液的基质效应,还原性物质对测定的干扰,脂浊及NBT的析出等导致体系混浊和不稳定提出了对策,并取得了满意的效果。结果通过对校正液生产工艺的改进,克服了基质效应,用2点速率法并将读数点尽量后延,消除了各种干扰。利用优选的非离子和阴离子表面活性剂,解决了体系的混浊和不稳定。经方法学评价,批内CV0.9%,日间CV2.9%,线性范围在0~6mmol/L,参考值范围为1.58~2.14mmol/L,试剂稳定1年。经临床核对6个批次共507例次的空腹血糖与果糖胺,结果完全符合。又观察了初诊确诊的糖尿病患者10例,核对了其果糖胺与空腹血糖和糖化血红蛋白的测定值,结果也完全符合。结论经改进的NBT法操作简便、试剂稳定、符合临床,适用于血清果糖胺的常规测定。更多还原

【Abstract】 Objective To modify nitroblue tetrazolium (NBT) reductive method for serum fructosamine (FMN) assay.Methods NBT assay has such principle problems as follows: matrix effects of calibration solution; interference from materials in samples; system clouding and unstablity due to lipid turbidity and NBT salting out in the system. Aiming at these problems, the corresponding strategy was introduced, which acquired a satisfied effect.Results The matrix effects of the calibrators have been overcame. And all other interferences were eliminated by using two point rate method and retardation of the window time. Turbidity of the system was omitted by using optimized anionic and nonionic detergents combined with salts of strong acid. Methodology evaluation: intra- and inter-assay precision CV were 0.9% and 2.9% respectively. Linearity range: 0-6 mmol/L; Assay rang: 1.58-2.14 mmol/L. The stability duration of the reagents was one year. In the clinical research trial, the level of FMN in 507 cases among 6 lots in two hospitals completely accorded with that of their respective fasting glucose. And at the same time, 10 initially diagnosed diabetes mellitus (DM) patients were observed. Their FMN also accorded with respective fasting glucose, and so did HbA1c.Conclusion The modified novel method is simple and stable, suitable for clinical routine measurement of serum FMN.更多还原