【摘要】 采用ICP-MS法测定动物血液和组织中硒,方法简便、快速、准确、检出限低。实验比较了样品消解方法、同位素的选择以及普通模式和ORS模式的差异,为同类样品的测定提供了参考。普通模式通过采用干扰方程,有效消除了82Kr+对82Se产生的干扰,选用82Se测定,测定结果准确可靠;ORS模式通过H2-碰撞反应池有效地消除了分子离子干扰,选用78Se测定,大大降低了检出限。普通模式的检出限为0.024ng.g-1,ORS模式的检出限为0.0046ng.g-1。两种方法的相对标准偏差均在1.8%~5.5%范围内,回收率为90.8%~107.2%。更多还原

【Abstract】 The Se in the blood and tissue of animal samples was determined by ICP-MS instrument. The method was not only proved simple and rapid, but also showed satisfying precision and low detection limit. The optimum conditions of ICP-MS for the method were investigated. Results by the digestion method, the isotopes selection, and the difference between the normal ICP-MS without ORS (Octopole Reaction System) and the ICP-MS with ORS were compared, which afford reference to the determination of the same kinds of sample. The standard materials were determined to proved the reliability of the normal ICP-MS and the ORS-ICP-MS methods. The ORS-ICP-MS can effectively remove the interference based on polyatomic ions, so the the detection limits were lowed, and the accuracy was improved. The detection limits of the method for normal ICP-MS is 0.024 ng·g-1 and that for ORS-ICP-MS is 0.004 6 ng·g-1, the RSDs are between 1.8% and 5.5%, and the recoveries of the method are in the range of 90.8%-107.2%.更多还原