【摘要】 通过PCR-SSCP技术检测了大通牦牛IGF-I基因的遗传多态性,对具有SSCP多态性的片段进行克隆和测序,找出等位基因的多态位点,将该位点多态性与大通牦牛生长性状进行相关性分析.结果显示:PCR-SSCP检测到大通牦牛具有AA、AB、BB 3种基因型,A(B)等位基因频率分别为0.902 8(0.097 2);对IGF-I基因第1内含子进行序列比对,发现一处单碱基C的缺失/插入,造成了该位点多态性的出现;6月龄和12月龄基因型为AA的大通牦牛的体质量和体斜长最小二乘均值显著高于AB和BB基因型(P<0.05);18月龄基因型为AB的大通牦牛的体高最小二乘均值显著高于BB型(P<0.05),说明大通牦牛品种中基因型为AA的个体生长性状有高于AB和BB基因型的趋势,可以作为大通牦牛品种选育的目标基因型.更多还原

【Abstract】 The polymorphism of IGF-I gene of Datong yak was detected by using PCR-SSCP technology,and the PCR products with polymorphism fragments were cloned and sequenced to detect single nucleotide polymorphism;the relationship between the polymorphism and Datong yak’s growth traits was analyzed.Results indicated that the frequences of allele genes A(B) is 0.902 8(0.097 2) in Datong yaks;the C-base-deletion in intron1 of IGF-I gene was detected by sequence align,causing polymorphism in Datong yaks;the body weight and body length of individuals with genotype AA at 6 and 12 months of age were significantly higher than those with genotype BB,AB(P<0.05);the body height of individuals with genotype AB at 18 months of age were significantly higher than those with genotype BB(P<0.05).The growth traits of individuals with genotype AA were significantly higher than those with genotype BB,AB in Datong yaks.This genotype could be selected as target-genotype in Datong yak.更多还原