【摘要】 用高效液相色谱法测定蒙药材肋柱花中小檗碱的含量。色谱流动相为乙腈∶水(V∶V=30∶70,每1000mL水中加入KH2PO43.4g),流速为0.8mL/min,紫外检测波长为345nm。结果表明,盐酸小檗碱在6.25—50μg/mL范围内具有良好的线性关系,相关系数为0.9992,实验测得平均回收率为97.7%,RSD=0.94%(n=6)。方法操作简便,灵敏度高,结果准确可靠。更多还原

【Abstract】 The RP-HPLC for determination of berberine hydrochloride in mongolian herbal drug Leizhuhua was established.Xterra (Sum×150mm×3.9mm.Waters) was used as chromatographic column, acetonitriLe-water (3∶7) as a mobile phase.The flow rate was 0.8mL/min and detection wavelength was at 345nm. The linear range of Berberine Hydrochloride was 6.25 —50μg/mL,r= 0.9992 .The average recovery rate was 97.7%,RSD=0.94%(n=6).The method is simple accurate and rapid with good reproducibility.更多还原