【摘要】 研究了四磺酸钠-1,6-己二胺二吖啶(TSHDA)与蛋白质的共振光散射增强作用,建立了一种新的蛋白质共振光散射分析体系。实验考察了吖啶探针浓度、缓冲体系pH对体系共振散射光的影响。在pH=3.0的酸性溶液中,蛋白质分子与TSHDA发生结合作用,共振光散射明显增强,最大散射波长位于460nm,体系散射强度随着探针浓度的增加而增大,蛋白质测定线性范围为0.2—1.2μg/mL,检出限9.7ng/mL。分析了血清蛋白合成样品,浓度为0.4—0.8μg/mL的样品测定回收率为97.5%—103.3%,相对标准偏差1.2%—2.6%。更多还原

【Abstract】 A sensitive RLS determination for protein has been developed based on the resonance light scattering of tetrasulfonate sodium-1,6-hexanediaminediacridine(TSHDA)with protein.The resonance light scattering of TSHDA could be greatly enhanced by protein with a resonance light scattering peak at 460 nm in pH 3.0.Effects of the concentration of TSHDA,pH value of the buffer solution were also studied and the optimum condition was gained.Under optimal conditions,a good calibration curve of the protein was obtained and the linear range of the calibration curve is 0.2—1.2μg/mL for albumin bovine(BSA),the detection limit is 9.7ng/mL-1.Applied to stimulant protein samples at the levels of 0.4,0.6 and 0.8 μg/mL of BSA,the recoveries were gained in the range of 97.5%—103.3% with the RSD of 1.2%—2.6%.This method is simple,rapid and sensitive.更多还原