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阻抑动力学光度法测定痕量槲皮素
Study on Kinetic Spectrophotometric Determination of Trace Quercetin
【摘要】 在氨性介质中,过氧化氢氧化次甲基蓝使其褪色,痕量槲皮素抑制该褪色反应,基于此建立了抑制动力学分光光度法测定槲皮素的新方法,方法的线性范围为1.5×10-5~4.5×10-4μg/mL,检出限为1.2×10-5μg/mL.
【Abstract】 A new kinetic spectrophotometric procedure for the analysis of Quercetin in Chinese traditional medicine has been described.This method was based on its catalytic effect of Quercetic on the redox reaction of methylene blue-hydrogen peroxide system in ammonium chloride cushion solution medium.The action of Quercetin in this system was studied.The linear range of determination is 1.5×10-5μg/ml-4.5×10-4μg/ml with detection limits of1.2×10-5μg/ml.The procedure was proved to be suitable in terms of selectivity for the micro amount of Quercetin in Chinese traditional medicine.
【关键词】 槲皮素;
次甲基蓝;
抑制作用;
抑制动力学分光光度法;
【Key words】 Quercetin; Methylene blue; Inhabitory Decoloration; Kinetic spectrophotometry;
【Key words】 Quercetin; Methylene blue; Inhabitory Decoloration; Kinetic spectrophotometry;
- 【文献出处】 赣南师范学院学报 ,Journal of Gannan Normal University , 编辑部邮箱 ,2008年06期
- 【分类号】R284
- 【下载频次】76