【摘要】 制备出了与蚕腺体内纺丝液浓度和后部丝腺pH值相近的再生丝素蛋白水溶液,利用静电纺丝技术模仿蚕在空气中吐丝的过程得到了再生丝素蛋白纤维。所得纤维的直径在350~4200nm之间,平均直径1700nm。通过正交实验方法考察了各种因素对纤维形态、直径及直径分布的影响,结果表明:所施电压对纤维的形态影响最大;溶液浓度对纤维的直径影响最大;喷头与接受板间距离对纤维直径的分布影响最大。实验得出了一个较好的静电纺丝条件为浓度30%,电压40kV,喷头与接受板间距离20cm。采用拉曼光谱、X衍射、DSC对再生丝素蛋白纤维进行了表征,发现再生丝素蛋白纤维中含有少量的Silk I结晶结构,主要以无定形结构为主,这与天然蚕丝的半结晶结构相比尚有差异。更多还原

【Abstract】 Regenerated silk fibroin aqueous solutions that concentration was similar in the silkworm’s spinning dope and pH value was the same pH value in the posterior division of the silkworm’s gland were prepared by a mild method.The regenerated B.mori.silk fibroin fibers were obtained by electrospinning with diameter range from 350-4200nm and an average diameter 1700nm.The effect of electrospinning parameters on the fiber morphology and fiber diameter of regenerated silk were investigated by orthogonal design.The result showed that voltage,the concentration of RSF solution and the distance between tip and collection plate were the most dominant parameter to fiber morphology,diameter and distribution,respectively.A optimal electrospinning condition was obtained for the concentration 30%,voltage 40kV,distance 20cm.These regeneration silk fibroin fibers were examined by Raman spectroscopy(RS),X-ray diffraction(XRD) and differential scanning calorimetry(DSC).It is found that the regeneration electrospun silk fibroin fibers involves a few silk Ⅰ crystalline structure,it was mainly an amorphous structure,this still have a big difference with the semicrystalline structure of natural B.mori.silk.更多还原