【摘要】 目的:研究胰岛素样生长因子-1(IGF-1)对大鼠局灶性脑缺血再灌注后Bcl-2表达的影响及与缺血时间关系,探讨IGF-1对脑缺血再灌注损伤的保护作用。方法:制作SD大鼠大脑中动脉缺血再灌注模型。将55只SD雄性大鼠随机分为假手术组(n=5)、对照组(n=25)、IGF-1治疗组(n=25),其中后两组按缺血再灌时间(6h,12h,1d,3d,7d)不同可分为5个亚组,每组5只,治疗组于缺血2h再灌注1h后经腹腔注入40μg/kg稀释为1ml的IGF-1,假手术组及对照组同时腹腔注入生理盐水1ml。以上动物均在再灌注后规定时间点用4%多聚甲醛经心脏灌注固定,取大鼠脑组织,应用免疫组化S-P法和HE染色检测Bcl-2蛋白表达及脑组织结构病理变化。结果:与对照组相比,治疗组大鼠脑组织Bcl-2表达明显增加,其神经细胞坏死程度明显减轻。结论:IGF-1在大鼠局灶性脑缺血再灌注损伤中起保护作用,其作用机制包括增加Bcl-2蛋白的表达,发挥其神经保护作用。更多还原

【Abstract】 Objective: To evaluate the protective effect of insulin-like growth factor-1(IGF-1) on focal cerebral ischemia reperfusion in rat.Methods: The healthy adult of male SD rats(55) were divided randomly into sham operation group(5),model group(25) and IGF-1 group(25),each of the model group and IGF-1 group was further divided into 5 subgroups according to the duration of reperfusion(6,12,24hours,3 day,7 day) after two hour of cerebral ischemia.Then the rats in sham operation and model group were injected normal saline.IGF-1 group were injected IGF-1 through abdominal cavity.Brain tissue of rats were used to observe nerve cell injury under light microscope after HE staining,and positive neurons of Bcl-2 were observed by immunohistochemical method.Results: Compared with the model group,the injury degree of neuron greatly reduced in the IGF-1 group(P<0.05).At the same time,the mumber of Bcl-2 positive neuron was increased significantly in IGF-1 group compared with model group(P<0.05).Conclusion: IGF-1 plays a protective role in rat focal cerebral ischemia-reperfusion injury,the nervous protective mechanism of IGF-1 may involve in increasing Bcl-2 activity.更多还原