【摘要】 对大花蕙兰的组织培养体系进行了研究。结果表明,在MS+1.0mg/L BA+0.4mg/LNAA培养基中培养25d后可诱导侧芽萌发;在MS+2.0 mg/L BA+2.0 mg/L IBA+1.0 mg/LGA培养基上增殖效果最好,增殖系数为3.62;当侧芽3cm左右时转入生根培养基1/2MS+1.0 mg/L IBA上进行生根及壮苗培养。将组培苗移入上层覆盖着树皮的基质中,移栽成活率达85%左右。更多还原

【Abstract】 Research on the establishment of in Vitro culture technology for Cymbidium grandiflorum is given in this article.The excised lateral buds begin to sprout on Murashige and Skoog(MS) basal medium containing 1.0mg/L 6-benzyladenine(BA) and 0.4mg/L naphthalene acetic acid(NAA) after 25 days in culture;Axenic explants on MS medium with 2.0mg/L BA,2.0mg/LNAA and 1.0 mg/LGA for propagation produce the highest propagation factor which is 3.62.The regenerated shoots of 3cm long are rooted and cultured in MS basal medium with 1.0mg/L 3.indole butyric acid(IBA).The axenic plants are transplanted to the base material covered with casca.The survival rate was about 85% for in vitro plant after transplantation.更多还原