【摘要】 目的:研究原发性闭角型青光眼虹膜基质胶原纤维含量的变化,为虹膜生物力学及闭角型青光眼发病机制的研究提供依据。方法:取排除内眼、外眼疾患的人眼根部虹膜11例,闭角型青光眼虹膜根部切除标本29例,置于40g/L甲醛溶液中固定48h,常规石蜡包埋,进行免疫组织化学染色。结果:Ⅰ,Ⅲ,Ⅳ型胶原在人眼虹膜中均有表达,其中I型胶原纤维在患者组表达减少,与正常组比较差异有显著性(P<0.05),Ⅲ,Ⅳ型胶原纤维的表达在两组无明显差异(P>0.05)。结论:原发性闭角型青光眼虹膜基质中Ⅰ型胶原纤维的表达减少提示其虹膜根部与正常眼相比具有较低的抗张能力,易导致周边虹膜向前膨隆而促发房角关闭。更多还原

【Abstract】 AIM:To study the changes of collagenous fibrils in iris of primary angle-closure glaucoma and to provide evidence for researches on iris biomechanics and pathogenesis of primary angle-closure glaucoma.METHODS: Normal iris root in 11 cases without oculopathy and primary angle-closure glaucoma iris root in 29 cases were fixed into 40g/L formalin for 48 hours, and then were embed ded by paraffin and observed by immunohistochemistry.RESULTS: Type Ⅰ,Ⅲ and Ⅳ collagen distributed in iris stroma and blood vessel wall. Type Ⅰ collagen was expressed lower in primary angle-closure glaucoma. There was no difference in the distribution of type Ⅲ or IV collagen between the two groups.CONCLUSION: The lower expression of type Ⅰ collagen indicates that the decrease of iris anti-tension ability may be involved in the pathogenesis of primary angle-closure glaucoma.更多还原