【摘要】 用改变培养条件的方法对海洋聚磷菌 Halomonas YSR-3的生长和除磷特性进行了研究。研究结果表明,无磷培养时,该菌菌体不能生长;用磷酸钾盐作为磷源时,菌体生长较好,形成多聚磷酸盐的菌体比例较高;较适合 YSR-3菌体生长和多聚磷酸盐形成的磷源是 KH₂PO₄,较适磷浓度为1 mmol/L。pH 的变化影响菌株的生长、多聚磷酸盐形成和除磷效果。pH 值为5时,菌体的数量几乎不增加(ΔOD₄₈₀为0.013),体内多聚磷酸盐和培养基中磷含量变化不大;pH 值为6、7和8时,菌体生长良好(ΔOD₄₈₀分别为1.529、1.539和1.497),95%以上的菌体内形成多聚磷酸盐,培养基中磷含量明显下降。YSR-3在不同培养基中除磷量和除磷率不同。在高磷培养基中除磷量为0.7 mmoL/L（磷含量由1.84mmoL/L 降到1.14 mmoL/L）,除磷率为37.5%;在低磷培养基中除磷量为0.02 mmol/L（磷含量由0.028 mmol/L 降到0.008 mmol/L）,除磷率为72.2%。更多还原

【Abstract】 The growth and the phosphorus absorption function of a marine bacterium Halomonas YSR-3 were studied by chang- ing its culture conditions.The results showed that in phosphorus-free medium,the ΔOD₄₈₀ of the cells was almost invari- able and the cells did not store intracellular polyphosphate granules （polyP）.YSR-3 grew better and had a higher ratio of the cells accumulating polyp in the medium containing sodium phosphate than in the medium containing potassium phos- phate.When the concentration of KH₂PO₄ was 1mmol/L,YSR-3 grew well and absorbed more phosphate with more intra- cellular polyP granules.When the medium’s pH value was 5,the stored intracellular polyP granules had not been metab- olized.However,when the pH value was 6,7 or 8,YSR-3 grew well and over 95% of the cells accumulated polyP, with the decreasing of phosphorus concentration in the supernatant.When YSR-3 was incubated in a high-phosphorus medium,the phosphorus concentration in the supematant decreased from 1.84 mmol/L to 1.14 mmol/L,absorbing 0.7 mmol/L phosphorus with a phosphorus-absorbing ratio of 37.5%;when YSR-3 was incubated in a low-phosphorus medi- um,the phosphorus concentration in the supernatant decreased from 0.028 mmol/L to 0.008 mmol/L,absorbing 0.02 mmol/L phosphorus with a phosphorus-absorbing ratio of 72.2%.更多还原