【摘要】 采用RT-PCR方法从小麦品种豫教2号的籽粒中克隆出淀粉合酶Ⅱ基因(Starch synthaseⅡ,SSⅡ)部分cDNA片段(600bp)(GenBank No.EF221761),同源性比较结果显示,它与GenBank上已报道的SSⅡ基因有高度同源性。以pCMBIA1301质粒为基础,构建了由35S启动子调控的SSⅡ基因的反义表达载体pCMBIA1301SSIIA;另外,还以pFGC5941质粒为基础,构建了SSⅡ基因的RNAi载体pFGC5941SSIIsa,这些载体的构建为研究此基因的功能奠定了基础。更多还原

【Abstract】 Starch synthase Ⅱ(SSⅡ) partial cDNA sequences(600bp)(GenBank No.EF221761) from grains of common wheat(Triticum aestivum,Yujiao 2 cultuvar) was amplified by RT-PCR.The result demonstrated that the cloned SSⅡ gene sequences were 99% identified with the reported SSⅡ genes in GenBank previously.In addition,its antisense expression vector was constructed with pCMBIA1301, and RNAi vector was also constructed with pFGC5941.These constructed vectors will provide a good background to study the function of SSⅡ on biosynthesis of starch in wheat plants.更多还原